Evaporated carbon stabilizes thin, frozen-hydrated specimens

Jakubowski, U.; Baumeister, W.; Glaeser, R. M.

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Evaporated carbon stabilizes thin, frozen-hydrated specimens

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Jakubowski, U., Baumeister, W., & Glaeser, R. M. (1989). Evaporated carbon stabilizes thin, frozen-hydrated specimens. Ultramicroscopy, 31(4), 351-356.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0010-7485-F

Abstract

A relatively simple modification has been designed and built for a commercial freeze-fracture machine which makes it possible to evaporate carbon onto frozen-hydrated specimens after the specimen grid has been mounted into a side-entry electron microscope cryo-holder. The evaporated carbon film greatly improves the stability of self-supported, vitreous ice films during exposure to the electron beam. Specimen charging is also greatly reduced. As a consequence, the image resolution attainable with carbon-coated, vitreous ice specimens appears to be limited only by the mechanical stability of the cryostage itself. (13 References).