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  Evaporated carbon stabilizes thin, frozen-hydrated specimens

Jakubowski, U., Baumeister, W., & Glaeser, R. M. (1989). Evaporated carbon stabilizes thin, frozen-hydrated specimens. Ultramicroscopy, 31(4), 351-356.

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Jakubowski, U.1, Author              
Baumeister, W.1, Author              
Glaeser, R. M., Author
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1External Organizations, ou_persistent22              

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Free keywords: Carbon; Electron microscopy; Specimen preparation; Sample preparation [A0660E]; Electron and ion microscopes and techniques [A0780]; Thin film growth, structure, and epitaxy [A6855]
 Abstract: A relatively simple modification has been designed and built for a commercial freeze-fracture machine which makes it possible to evaporate carbon onto frozen-hydrated specimens after the specimen grid has been mounted into a side-entry electron microscope cryo-holder. The evaporated carbon film greatly improves the stability of self-supported, vitreous ice films during exposure to the electron beam. Specimen charging is also greatly reduced. As a consequence, the image resolution attainable with carbon-coated, vitreous ice specimens appears to be limited only by the mechanical stability of the cryostage itself. (13 References).

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 Dates: 1989
 Publication Status: Published in print
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 Identifiers: eDoc: 318480
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Title: Ultramicroscopy
Source Genre: Journal
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Pages: - Volume / Issue: 31 (4) Sequence Number: - Start / End Page: 351 - 356 Identifier: -