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  Characterization of Tau in cerebrospinal fluid using mass spectrometry

Portelius, E., Hansson, S. F., Tran, A. J., Zetterberg, H., Grognet, P., Vanmechelen, E., et al. (2008). Characterization of Tau in cerebrospinal fluid using mass spectrometry. Journal of Proteome Reserach, 7(2), 2114-2120. doi:10.1021/pr7008669.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7FF4-E Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7FF5-C
Genre: Journal Article
Alternative Title : J Proteome Res

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 Creators:
Portelius, Erik, Author
Hansson, Sara F., Author
Tran, Ai Jun, Author
Zetterberg, Henrik, Author
Grognet, Pierre, Author
Vanmechelen, Eugeen, Author
Höglund, Kina, Author
Brinkmalm, Gunnar, Author
Westman-Brinkmalm, Ann, Author
Nordhoff, Eckhard1, Author              
Blennow, Kaj, Author
Gobom, Johan1, Author              
Affiliations:
1Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

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 Abstract: The neurodegenerative disorder Alzheimer’s disease (AD) is the most common cause of dementia in the elderly. The presence of neurofibrillary tangles, consisting of hyperphosphorylated tau protein, is one of the major neuropathologic characteristics of the disease, making this protein an attractive biomarker for AD and a possible target for therapy. Here, we describe an optimized immunoprecipitation mass spectrometry method that enables, for the first time, detailed characterization of tau in human cerebrospinal fluid. The identities of putative tau fragments were confirmed using nanoflow liquid chromatography and tandem mass spectrometry. Nineteen tryptic fragments of tau were detected, of which 16 are found in all tau isoforms while 3 represented unique tau isoforms. These results pave the way for clinical CSF studies on the tauopathies.

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Language(s): eng - English
 Dates: 2008-05-02
 Publication Status: Published in print
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Title: Journal of Proteome Reserach
  Alternative Title : J Proteome Res
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 7 (2) Sequence Number: - Start / End Page: 2114 - 2120 Identifier: ISSN: 1535-3893