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  Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples

Boersema, P. J., Geiger, T., Wisniewski, J. R., & Mann, M. (2013). Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. MOLECULAR & CELLULAR PROTEOMICS, 12(1), 158-171. doi:10.1074/mcp.M112.023614.

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Boersema, Paul Jonathan1, Author           
Geiger, Tamar1, Author           
Wisniewski, Jacek R.1, Author           
Mann, Matthias1, Author           
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1Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              

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Free keywords: CHROMATOGRAPHY MASS-SPECTROMETRY; DISEASE FLUID PROTEIN; HUMAN PLASMA PROTEOME; QUANTITATIVE PROTEOMICS; GLYCOPEPTIDE CAPTURE; CELL-LINES; EXPRESSION; IDENTIFICATION; GLYCOPROTEINS; BIOMARKERS
 Abstract: Cells secrete a large number of proteins to communicate with their surroundings. Furthermore, plasma membrane proteins and intracellular proteins can be released into the extracellular space by regulated or non-regulated processes. Here, we profiled the supernatant of 11 cell lines that are representative of different stages of breast cancer development by specifically capturing N-glycosylated peptides using the N-glyco FASP technology. For accurate quantification we developed a super-SILAC mix from several labeled breast cancer cell lines and used it as an internal standard for all samples. In total, 1398 unique N-glycosylation sites were identified and quantified. Enriching for N-glycosylated peptides focused the analysis on classically secreted and membrane proteins. N-glycosylated secretome profiles correctly clustered the different cell lines to their respective cancer stage, suggesting that biologically relevant differences were detected. Five different profiles of glycoprotein dynamics during cancer development were detected, and they contained several proteins with known roles in breast cancer. We then used the super-SILAC mix in plasma, which led to the quantification of a large number of the previously identified N-glycopeptides in this important body fluid. The combination of quantifying the secretome of cancer cell lines and of human plasma with a super-SILAC approach appears to be a promising new approach for finding markers of disease. Molecular & Cellular Proteomics 12: 10.1074/mcp.M112.023614, 158-171, 2013.

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Language(s): eng - English
 Dates: 2013-01
 Publication Status: Issued
 Pages: 14
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000313557600012
DOI: 10.1074/mcp.M112.023614
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Title: MOLECULAR & CELLULAR PROTEOMICS
Source Genre: Journal
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Publ. Info: 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA : AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Pages: - Volume / Issue: 12 (1) Sequence Number: - Start / End Page: 158 - 171 Identifier: ISSN: 1535-9476