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  Correlated stage- and subfield-associated hippocampal gene expression patterns in experimental and human temporal lobe epilepsy

Becker, A., Chen, J., Zien, A., Sochivko, D., Normann, S., Schramm, J., et al. (2003). Correlated stage- and subfield-associated hippocampal gene expression patterns in experimental and human temporal lobe epilepsy. European Journal of Neuroscience: European Neuroscience Association, 18(10), 2792-2802. doi:10.1111/j.1460-9568.2003.02993.x.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-DAC1-F Version Permalink: http://hdl.handle.net/21.11116/0000-0005-6933-C
Genre: Journal Article

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 Creators:
Becker, AJ, Author
Chen, J, Author
Zien, A1, 2, Author              
Sochivko, D, Author
Normann , S, Author
Schramm, J, Author
Elger, CE, Author
Wiesler, OD, Author
Blumcke, I, Author
Affiliations:
1Department Empirical Inference, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497795              
2Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497794              

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 Abstract: Epileptic activity evokes profound alterations of hippocampal organization and function. Genomic responses may reflect immediate consequences of excitatory stimulation as well as sustained molecular processes related to neuronal plasticity and structural remodeling. Using oligonucleotide microarrays with 8799 sequences, we determined subregional gene expression profiles in rats subjected to pilocarpine-induced epilepsy (U34A arrays, Affymetrix, Santa Clara, CA, USA; P < 0.05, twofold change, n = 3 per stage). Patterns of gene expression corresponded to distinct stages of epilepsy development. The highest number of differentially expressed genes (dentate gyrus, approx. 400 genes and CA1, approx. 700 genes) was observed 3 days after status epilepticus. The majority of up-regulated genes was associated with mechanisms of cellular stress and injury - 14 days after status epilepticus, numerous transcription factors and genes linked to cytoskeletal and synaptic reorganization were differentially expressed and, in the stage of chronic spontaneous seizures, distinct changes were observed in the transcription of genes involved in various neurotransmission pathways and between animals with low vs. high seizure frequency. A number of genes (n = 18) differentially expressed during the chronic epileptic stage showed corresponding expression patterns in hippocampal subfields of patients with pharmacoresistant temporal lobe epilepsy (n = 5 temporal lobe epilepsy patients; U133A microarrays, Affymetrix; covering 22284 human sequences). These data provide novel insights into the molecular mechanisms of epileptogenesis and seizure-associated cellular and structural remodeling of the hippocampus.

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 Dates: 2003-11
 Publication Status: Published in print
 Pages: -
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 Rev. Method: -
 Identifiers: BibTex Citekey: 2501
DOI: 10.1111/j.1460-9568.2003.02993.x
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Title: European Journal of Neuroscience : European Neuroscience Association
  Other : Eur. J. Neurosci
Source Genre: Journal
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Publ. Info: Oxford, UK : Published on behalf of the European Neuroscience Association by Oxford University Press
Pages: - Volume / Issue: 18 (10) Sequence Number: - Start / End Page: 2792 - 2802 Identifier: ISSN: 0953-816X
CoNE: https://pure.mpg.de/cone/journals/resource/954925575988