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  Coaligned dual-channel STED nanoscopy and molecular diffusion analysis at 20 nm resolution.

Göttfert, F., Wurm, C. A., Müller, V., Berning, S., Cordes, V. C., Honigmann, A., et al. (2013). Coaligned dual-channel STED nanoscopy and molecular diffusion analysis at 20 nm resolution. Biophysical Journal, 105(1), L01-L03. doi:10.1016/j.bpj.2013.05.029.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-E53F-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-3A77-4
Genre: Journal Article

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1795834.pdf (Publisher version), 713KB
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 Creators:
Göttfert, F.1, Author              
Wurm, C. A.1, Author              
Müller, V.1, Author              
Berning, S.1, Author              
Cordes, V. C.2, Author              
Honigmann, A.1, Author              
Hell, S. W.1, Author              
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              
2Research Group of Nuclear Architecture, MPI for biophysical chemistry, Max Planck Society, ou_578575              

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 Abstract: We report on a fiber laser-based stimulated emission-depletion microscope providing down to ~20 nm resolution in raw data images as well as 15–19 nm diameter probing areas in fluorescence correlation spectroscopy. Stimulated emission depletion pulses of nanosecond duration and 775 nm wavelength are used to silence two fluorophores simultaneously, ensuring offset-free colocalization analysis. The versatility of this superresolution method is exemplified by revealing the octameric arrangement of Xenopus nuclear pore complexes and by quantifying the diffusion of labeled lipid molecules in artificial and living cell membranes.

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Language(s): eng - English
 Dates: 2013-07-022013
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.bpj.2013.05.029
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Title: Biophysical Journal
Source Genre: Journal
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Pages: - Volume / Issue: 105 (1) Sequence Number: - Start / End Page: L01 - L03 Identifier: -