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  A Topological Map of the Compartmentalized Arabidopsis thaliana Leaf Metabolome

Krueger, S., Giavalisco, P., Krall, L., Steinhauser, M.-C., Buessis, D., Usadel, B., et al. (2011). A Topological Map of the Compartmentalized Arabidopsis thaliana Leaf Metabolome. PLoS One, 6(3), e17806. doi:10.1371/journal.pone.0017806.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2192-0 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2193-E
Genre: Journal Article

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 Creators:
Krueger, S.1, Author
Giavalisco, P.2, Author              
Krall, L.3, Author              
Steinhauser, M.-C.4, Author              
Buessis, D.5, Author              
Usadel, B.6, Author              
Fluegge, U.-I.1, Author
Fernie, A. R.7, Author              
Willmitzer, L.3, Author              
Steinhauser, D.3, Author              
Affiliations:
1External Organizations, ou_persistent22              
2Experimental Systems Biology, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753342              
3Small Molecules, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753340              
4System Regulation, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753327              
5Developmental Physiology and Genomics, Cooperative Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753313              
6Integrative Carbon Biology, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753329              
7Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

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Free keywords: spectrometry-based metabolomics mass-spectrometry spinach leaves potato-tubers higher-plants intracellular-localization glucose pyrophosphorylase epithiospecifier protein subcellular-localization nonaqueous fractionation
 Abstract: Background: The extensive subcellular compartmentalization of metabolites and metabolism in eukaryotic cells is widely acknowledged and represents a key factor of metabolic activity and functionality. In striking contrast, the knowledge of actual compartmental distribution of metabolites from experimental studies is surprisingly low. However, a precise knowledge of, possibly all, metabolites and their subcellular distributions remains a key prerequisite for the understanding of any cellular function. Methodology/Principal Findings: Here we describe results for the subcellular distribution of 1,117 polar and 2,804 lipophilic mass spectrometric features associated to known and unknown compounds from leaves of the model plant Arabidopsis thaliana. Using an optimized non-aqueous fractionation protocol in conjunction with GC/MS- and LC/MS-based metabolite profiling, 81.5% of the metabolic data could be associated to one of three subcellular compartments: the cytosol (including the mitochondria), vacuole, or plastids. Statistical analysis using a marker-'free' approach revealed that 18.5% of these metabolites show intermediate distributions, which can either be explained by transport processes or by additional subcellular compartments. Conclusion/Significance: Next to a functional and conceptual workflow for the efficient, highly resolved metabolite analysis of the fractionated Arabidopsis thaliana leaf metabolome, a detailed survey of the subcellular distribution of several compounds, in the graphical format of a topological map, is provided. This complex data set therefore does not only contain a rich repository of metabolic information, but due to thorough validation and testing by statistical methods, represents an initial step in the analysis of metabolite dynamics and fluxes within and between subcellular compartments.

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Language(s): eng - English
 Dates: 2011-03-152011
 Publication Status: Published in print
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 Table of Contents: -
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 Identifiers: ISI: ISI:000288513900033
DOI: 10.1371/journal.pone.0017806
ISSN: 1932-6203
URI: ://000288513900033 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3058050/pdf/pone.0017806.pdf?tool=pmcentrez
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Title: PLoS One
Source Genre: Journal
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Pages: - Volume / Issue: 6 (3) Sequence Number: - Start / End Page: e17806 Identifier: -