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  Transcript and metabolite profiling of the adaptive response to mild decreases in oxygen concentration in the roots of arabidopsis plants

van Dongen, J. T., Frohlich, A., Ramirez-Aguilar, S. J., Schauer, N., Fernie, A. R., Erban, A., et al. (2009). Transcript and metabolite profiling of the adaptive response to mild decreases in oxygen concentration in the roots of arabidopsis plants. Annals of Botany, 103(2), 269-280. doi:10.1093/aob/mcn126.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-24B1-0 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-84EA-4
Genre: Journal Article

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 Creators:
van Dongen, J. T.1, Author              
Frohlich, A.2, Author
Ramirez-Aguilar, S. J.1, Author              
Schauer, N.3, Author              
Fernie, A. R.3, Author              
Erban, A.4, Author              
Kopka, J.4, Author              
Clark, J.2, Author
Langer, A.2, Author
Geigenberger, P.5, Author              
Affiliations:
1Energy Metabolism, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753325              
2External Organizations, ou_persistent22              
3Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              
4Applied Metabolome Analysis, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753338              
5Storage Carbohydrate Metabolism, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753336              

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Free keywords: growing potato-tubers low internal oxygen genome-wide analysis mass-spectrometry gas-chromatography storage metabolism gene-expression energy status seedlings system
 Abstract: Oxygen can fall to low concentrations within plant tissues, either because of environmental factors that decrease the external oxygen concentration or because the movement of oxygen through the plant tissues cannot keep pace with the rate of oxygen consumption. Recent studies document that plants can decrease their oxygen consumption in response to relatively small changes in oxygen concentrations to avoid internal anoxia. The molecular mechanisms underlying this response have not been identified yet. The aim of this study was to use transcript and metabolite profiling to investigate the genomic response of arabidopsis roots to a mild decrease in oxygen concentrations. Arabidopsis seedlings were grown on vertical agar plates at 21, 8, 4 and 1 % (v/v) external oxygen for 0.5, 2 and 48 h. Roots were analysed for changes in transcript levels using Affymetrix whole genome DNA microarrays, and for changes in metabolite levels using routine GC-MS based metabolite profiling. Root extension rates were monitored in parallel to investigate adaptive changes in growth. The results show that root growth was inhibited and transcript and metabolite profiles were significantly altered in response to a moderate decrease in oxygen concentrations. Low oxygen leads to a preferential up-regulation of genes that might be important to trigger adaptive responses in the plant. A small but highly specific set of genes is induced very early in response to a moderate decrease in oxygen concentrations. Genes that were down-regulated mainly encoded proteins involved in energy-consuming processes. In line with this, root extension growth was significantly decreased which will ultimately save ATP and decrease oxygen consumption. This was accompanied by a differential regulation of metabolite levels at short- and long-term incubation at low oxygen. The results show that there are adaptive changes in root extension involving large-scale reprogramming of gene expression and metabolism when oxygen concentration is decreased in a very narrow range.

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Language(s): eng - English
 Dates: 2008-07-292009
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: ISI: ISI:000262329200013
DOI: 10.1093/aob/mcn126
ISSN: 1095-8290 (Electronic)0305-7364 (Linking)
URI: ://000262329200013http://aob.oxfordjournals.org/content/103/2/269.full.pdf
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Title: Annals of Botany
Source Genre: Journal
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Publ. Info: London : Elsevier
Pages: - Volume / Issue: 103 (2) Sequence Number: - Start / End Page: 269 - 280 Identifier: ISSN: 0305-7364
CoNE: https://pure.mpg.de/cone/journals/resource/110992357367384