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  Tissue-specific down-regulation of LjAMT1;1 compromises nodule function and enhances nodulation in Lotus japonicus

Rogato, A., D'Apuzzo, E., Barbulova, A., Omrane, S., Stedel, C., Simon-Rosin, U., et al. (2008). Tissue-specific down-regulation of LjAMT1;1 compromises nodule function and enhances nodulation in Lotus japonicus. Plant Molecular Biology, 68(6), 585-595. doi:10.1007/s11103-008-9394-5.

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Rogato-2008-Tissue-specific down.pdf (beliebiger Volltext), 578KB
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Rogato, A.1, Autor
D'Apuzzo, E.1, Autor
Barbulova, A.1, Autor
Omrane, S.1, Autor
Stedel, C.1, Autor
Simon-Rosin, U.2, 3, Autor           
Katinakis, P.1, Autor
Flemetakis, M.1, Autor
Udvardi, M. K.2, Autor           
Chiurazzi, M.1, Autor
Affiliations:
1External Organizations, ou_persistent22              
2Molecular Plant Nutrition, Max Planck Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753321              
3Biophysical Analysis, Infrastructure Groups and Service Units, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753307              

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Schlagwörter: ammonium transport nitrogen fixation nodule symbiosis affinity ammonium transporters nitrogen-fixation root-nodules phosphoenolpyruvate carboxylase peribacteroid membrane symbiosome membrane soybean nodules legume nodules plant expression
 Zusammenfassung: Plant ammonium transporters of the AMT1 family are involved in N-uptake from the soil and ammonium transport, and recycling within the plant. Although AMT1 genes are known to be expressed in nitrogen-fixing nodules of legumes, their precise roles in this specialized organ remain unknown. We have taken a reverse-genetic approach to decipher the physiological role of LjAMT1;1 in Lotus japonicus nodules. LjAMT1;1 is normally expressed in both the infected zone and the vascular tissue of Lotus nodules. Inhibition of LjAMT1;1 gene expression, using an antisense gene construct driven by a leghemoglobin promoter resulted in a substantial reduction of LjAMT1;1 transcript in the infected tissue but not the vascular bundles of transgenic plants. As a result, the nitrogen-fixing activity of nodules was partially impaired and nodule number increased compared to control plants. Expression of LjAMT1;1-GFP fusion protein in plant cells indicated a plasma-membrane location for the LjAMT1;1 protein. Taken together, the results are consistent with a role of LjAMT1;1 in retaining ammonium derived from symbiotic nitrogen fixation in plant cells prior to its assimilation.

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Sprache(n): eng - English
 Datum: 2008-09-112008
 Publikationsstatus: Erschienen
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 Identifikatoren: ISI: ISI:000260378600005
DOI: 10.1007/s11103-008-9394-5
ISSN: 0167-4412 (Print) 0167-4412 (Linking)
URI: ://000260378600005 http://www.springerlink.com/content/kl8037857414pk8r/fulltext.pdf
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Titel: Plant Molecular Biology
Genre der Quelle: Zeitschrift
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Seiten: - Band / Heft: 68 (6) Artikelnummer: - Start- / Endseite: 585 - 595 Identifikator: -