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  The influence of cytosolic phosphorylating glyceraldehyde 3-phosphate dehydrogenase (GAPC) on potato tuber metabolism

Hajirezaei, M.-R., Biemelt, S., Peisker, M., Lytovchenko, A., Fernie, A. R., & Sonnewald, U. (2006). The influence of cytosolic phosphorylating glyceraldehyde 3-phosphate dehydrogenase (GAPC) on potato tuber metabolism. Journal of Experimental Botany, 57(10), 2363-2377. doi:10.1093/jxb/erj207.

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Hajirezaei, M.-R.1, Author
Biemelt, S.1, Author
Peisker, M.1, Author
Lytovchenko, A.2, Author              
Fernie, A. R.2, Author              
Sonnewald, U.1, Author
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1External Organizations, ou_persistent22              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

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Free keywords: antisense carbohydrate metabolism gapdh potato tuber adp-glucose pyrophosphorylase increased adpglucose pyrophosphorylase chromatography-mass spectrometry severe growth-retardation solanum-tuberosum plant-tissues carbohydrate-metabolism antisense repression heterotrophic cells carbon metabolism
 Abstract: The aim of this work was to investigate the importance of cytosolic phosphorylating glyceralclehyde 3-phosphate dehydrogenase (GAPC) in potato carbohydrate metabolism. For this purpose, the cytosolic isoform of phosphorylating GAPC was cloned and used for an antisense approach to generate transgenic potato plants that exhibited constitutively decreased GAPDH activity. Potato lines with decreased activities of phosphorylating GAPC exhibited no major changes in either whole-plant or tuber morphology. However, the levels of 3-phosphoglycerate were decreased in leaves of the transformants. A broad metabolic phenotyping of tubers from the transformants revealed an increase in sucrose and UDPgiucose content, a decrease in the glycolytic intermediates 3-phosphoglycerate and phosphoenolpyruvate but little change in the levels of other metabolites. Moreover, the transformants displayed no differences in cold sweetening with respect to the wild type. Taken together these data suggest that phosphorylating GAPC plays only a minor role in the regulation of potato metabolism. The results presented here are discussed in relation to current models regarding primary metabolism in the potato tuber parenchyma.

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Language(s): eng - English
 Dates: 2006-06-272006
 Publication Status: Published in print
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 Table of Contents: -
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 Identifiers: ISI: ISI:000239389700022
DOI: 10.1093/jxb/erj207
ISSN: 0022-0957 (Print) 0022-0957 (Linking)
URI: ://000239389700022 http://jxb.oxfordjournals.org/content/57/10/2363.full.pdf
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Title: Journal of Experimental Botany
Source Genre: Journal
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Pages: - Volume / Issue: 57 (10) Sequence Number: - Start / End Page: 2363 - 2377 Identifier: -