非表示:
キーワード:
antisense
carbohydrate metabolism
gapdh
potato tuber
adp-glucose pyrophosphorylase
increased adpglucose pyrophosphorylase
chromatography-mass spectrometry
severe growth-retardation
solanum-tuberosum
plant-tissues
carbohydrate-metabolism
antisense repression
heterotrophic cells
carbon metabolism
要旨:
The aim of this work was to investigate the importance of cytosolic phosphorylating glyceralclehyde 3-phosphate dehydrogenase (GAPC) in potato carbohydrate metabolism. For this purpose, the cytosolic isoform of phosphorylating GAPC was cloned and used for an antisense approach to generate transgenic potato plants that exhibited constitutively decreased GAPDH activity. Potato lines with decreased activities of phosphorylating GAPC exhibited no major changes in either whole-plant or tuber morphology. However, the levels of 3-phosphoglycerate were decreased in leaves of the transformants. A broad metabolic phenotyping of tubers from the transformants revealed an increase in sucrose and UDPgiucose content, a decrease in the glycolytic intermediates 3-phosphoglycerate and phosphoenolpyruvate but little change in the levels of other metabolites. Moreover, the transformants displayed no differences in cold sweetening with respect to the wild type. Taken together these data suggest that phosphorylating GAPC plays only a minor role in the regulation of potato metabolism. The results presented here are discussed in relation to current models regarding primary metabolism in the potato tuber parenchyma.
