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  Structure determination of an 11-subunit exosome in complex with RNA by molecular replacement

Makino, D. L., & Conti, E. (2013). Structure determination of an 11-subunit exosome in complex with RNA by molecular replacement. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 69, 2226-2235. doi:10.1107/S0907444913011438.

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Makino, Debora Lika1, Author           
Conti, Elena1, Author           
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1Conti, Elena / Structural Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565144              

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Free keywords: EUKARYOTIC EXOSOME; ARCHAEAL EXOSOME; CORE; DEGRADATION; RECONSTITUTION; MECHANISM; MULTIPLE; SUBUNITexosome; molecular replacement; model building; RNA; nucleases; Rrp44;
 Abstract: The RNA exosome is an evolutionarily conserved multi-protein complex involved in the 3 degradation of a variety of RNA transcripts. In the nucleus, the exosome participates in the maturation of structured RNAs, in the surveillance of pre-mRNAs and in the decay of a variety of noncoding transcripts. In the cytoplasm, the exosome degrades mRNAs in constitutive and regulated turnover pathways. Several structures of subcomplexes of eukaryotic exosomes or related prokaryotic exosome-like complexes are known, but how the complete assembly is organized to fulfil processive RNA degradation has been unclear. An atomic snapshot of a Saccharomyces cerevisiae 420kDa exosome complex bound to an RNA substrate in the pre-cleavage state of a hydrolytic reaction has been determined. Here, the crystallographic steps towards the structural elucidation, which was carried out by molecular replacement, are presented.

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Language(s): eng - English
 Dates: 2013-11
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000326648900009
DOI: 10.1107/S0907444913011438
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Title: ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
Source Genre: Journal
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Publ. Info: 111 RIVER ST, HOBOKEN 07030-5774, NJ USA : WILEY-BLACKWELL
Pages: - Volume / Issue: 69 Sequence Number: - Start / End Page: 2226 - 2235 Identifier: ISSN: 0907-4449