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  The first transformation method for the thermo-acidophilic archaeon Thermoplasma acidophilum

Baka, E., Varga, S., Hobel, C., Knispel, R. W., Fekete, C., Ivanics, M., et al. (2013). The first transformation method for the thermo-acidophilic archaeon Thermoplasma acidophilum. JOURNAL OF MICROBIOLOGICAL METHODS, 95(2), 145-148. doi:10.1016/j.mimet.2013.08.006.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-D109-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-D10A-6
Genre: Journal Article

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 Creators:
Baka, Erzsebet1, Author
Varga, Sandor1, Author
Hobel, Cedric1, Author
Knispel, Roland Wilhelm2, Author              
Fekete, Csaba1, Author
Ivanics, Milan1, Author
Kriszt, Balazs1, Author
Nagy, Istvan2, Author              
Kukolya, Jozsef1, Author
Affiliations:
1external, ou_persistent22              
2Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

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Free keywords: HOMOLOGOUS RECOMBINATION; DEOXYRIBONUCLEIC-ACID; GENE DISRUPTION; EXOGENOUS DNA; SULFOLOBUS; GENOME; VECTORS; SIZEArchaea; Novobiocin; Plasmid; Thermoplasma acidophilum; Transformation; Homologous recombination;
 Abstract: A transformation method yielding up to 10(4) transformants per mu g circular DNA was developed for Thermoplasma acidophilum. The method is based on a natural DNA uptake process in which T. acidophilum cells keep their integrity and turn competent at pH 3.5 and 58 degrees C. Shuttle vector maintenance could not be detected, since the used Nov(R) gyraseB gene integrated into its chromosomal counterpart by homologous recombination. (C) 2013 Elsevier B.V. All rights reserved.

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Language(s): eng - English
 Dates: 2013-11
 Publication Status: Published in print
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: JOURNAL OF MICROBIOLOGICAL METHODS
Source Genre: Journal
 Creator(s):
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Publ. Info: PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS : ELSEVIER SCIENCE BV
Pages: - Volume / Issue: 95 (2) Sequence Number: - Start / End Page: 145 - 148 Identifier: ISSN: 0167-7012