The first transformation method for the thermo-acidophilic archaeon 
Thermoplasma acidophilum

Baka, Erzsebet; Varga, Sandor; Hobel, Cedric; Knispel, Roland Wilhelm; Fekete, Csaba; Ivanics, Milan; Kriszt, Balazs; Nagy, Istvan; Kukolya, Jozsef

doi:10.1016/j.mimet.2013.08.006

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The first transformation method for the thermo-acidophilic archaeon Thermoplasma acidophilum

Baka, E., Varga, S., Hobel, C., Knispel, R. W., Fekete, C., Ivanics, M., et al. (2013). The first transformation method for the thermo-acidophilic archaeon Thermoplasma acidophilum. JOURNAL OF MICROBIOLOGICAL METHODS, 95(2), 145-148. doi:10.1016/j.mimet.2013.08.006.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0014-D109-8 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0014-D10A-6

Genre: Journal Article

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Creators:
Baka, Erzsebet¹, Author
Varga, Sandor¹, Author
Hobel, Cedric¹, Author
Knispel, Roland Wilhelm², Author
Fekete, Csaba¹, Author
Ivanics, Milan¹, Author
Kriszt, Balazs¹, Author
Nagy, Istvan², Author
Kukolya, Jozsef¹, Author

Affiliations:
1external, ou_persistent22
2Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142

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Free keywords: HOMOLOGOUS RECOMBINATION; DEOXYRIBONUCLEIC-ACID; GENE DISRUPTION; EXOGENOUS DNA; SULFOLOBUS; GENOME; VECTORS; SIZEArchaea; Novobiocin; Plasmid; Thermoplasma acidophilum; Transformation; Homologous recombination;

Abstract: A transformation method yielding up to 10(4) transformants per mu g circular DNA was developed for Thermoplasma acidophilum. The method is based on a natural DNA uptake process in which T. acidophilum cells keep their integrity and turn competent at pH 3.5 and 58 degrees C. Shuttle vector maintenance could not be detected, since the used Nov(R) gyraseB gene integrated into its chromosomal counterpart by homologous recombination. (C) 2013 Elsevier B.V. All rights reserved.

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Language(s): eng - English

Dates: Date issued: 2013-11

Publication Status: Issued

Pages: 4

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Table of Contents: -

Rev. Type: Peer

Identifiers: ISI: 000327805600008
DOI: 10.1016/j.mimet.2013.08.006

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Title: JOURNAL OF MICROBIOLOGICAL METHODS

Source Genre: Journal

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Publ. Info: PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS : ELSEVIER SCIENCE BV

Pages: - Volume / Issue: 95 (2) Sequence Number: - Start / End Page: 145 - 148 Identifier: ISSN: 0167-7012