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  High-throughput method for determination of seed paternity by microsatellite markers

Bhattacharya, S., & Baldwin, I. T. (2013). High-throughput method for determination of seed paternity by microsatellite markers. Bio-protocol, 3(8), 1-12. doi:10.21769/BioProtoc.452.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0014-F4C6-0 Version Permalink: https://hdl.handle.net/21.11116/0000-0007-1271-5
Genre: Journal Article

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 Creators:
Bhattacharya, Samik1, Author           
Baldwin, Ian Thomas1, Author           
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1Department of Molecular Ecology, Prof. I. T. Baldwin, MPI for Chemical Ecology, Max Planck Society, ou_24029              

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 Abstract: In this protocol, determination of seed paternity by microsatellite markers in Nicotiana attenuata is described. However, this does not include a protocol for the novel marker selection/identification, but rather exploits the markers generated for a closely related species N. tabacum (Bindler et al., 2007). This is a high-throughput protocol optimized and streamlined for one skilled person to process 384 (96 x 4) seeds in 5 days, from DNA isolation (from seedlings) to paternity assessment by microsatellite genotype data.

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 Dates: 201320132013
 Publication Status: Issued
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 Identifiers: Other: ITB450
DOI: 10.21769/BioProtoc.452
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Title: Bio-protocol
Source Genre: Journal
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Publ. Info: Berkley : Bio Online
Pages: - Volume / Issue: 3 (8) Sequence Number: - Start / End Page: 1 - 12 Identifier: ISSN: 2331-8325
CoNE: https://pure.mpg.de/cone/journals/resource/2331-8325