English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
  Local TagsRelease HistoryDetailsSummary

Released
Journal Article

High-throughput method for determination of seed paternity by microsatellite markers

MPS-Authors
/persons/resource/persons3807

Bhattacharya,  Samik
Department of Molecular Ecology, Prof. I. T. Baldwin, MPI for Chemical Ecology, Max Planck Society;

/persons/resource/persons3786

Baldwin,  Ian Thomas
Department of Molecular Ecology, Prof. I. T. Baldwin, MPI for Chemical Ecology, Max Planck Society;

External Resource

http://www.bio-protocol.org/e452
(Publisher version)

Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)

ITB450.pdf
(Publisher version), 862KB

Supplementary Material (public)
There is no public supplementary material available
Citation

Bhattacharya, S., & Baldwin, I. T. (2013). High-throughput method for determination of seed paternity by microsatellite markers. Bio-protocol, 3(8), 1-12. doi:10.21769/BioProtoc.452.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0014-F4C6-0
Abstract
In this protocol, determination of seed paternity by microsatellite markers in Nicotiana attenuata is described. However, this does not include a protocol for the novel marker selection/identification, but rather exploits the markers generated for a closely related species N. tabacum (Bindler et al., 2007). This is a high-throughput protocol optimized and streamlined for one skilled person to process 384 (96 x 4) seeds in 5 days, from DNA isolation (from seedlings) to paternity assessment by microsatellite genotype data.