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  Caspases mediate nucleoporin cleavage, but not early redistribution of nuclear transport factors and modulation of nuclear permeability in apoptosis.

Ferrando-May, E., Cordes, V., Biller-Ckovric, I., Mirkovic, J., Görlich, D., & Nicotera, P. (2001). Caspases mediate nucleoporin cleavage, but not early redistribution of nuclear transport factors and modulation of nuclear permeability in apoptosis. Cell Death and Differentiation, 8(5), 495-505.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0015-3D6C-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-8993-9
Genre: Journal Article

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1932939.pdf (Publisher version), 361KB
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 Creators:
Ferrando-May, E., Author
Cordes, V., Author
Biller-Ckovric, I., Author
Mirkovic, J., Author
Görlich, D.1, Author              
Nicotera, P., Author
Affiliations:
1Department of Cellular Logistics, MPI for biophysical chemistry, Max Planck Society, ou_578574              

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Free keywords: caspase; nuclear pore complex; nuclear import; mRNA export; microinjection
 Abstract: In eukaryotic cells, both soluble transport factors and components of the nuclear pore complex mediate protein and RNA trafficking between the nucleus and the cytoplasm. Here, we investigated whether caspases, the major execution system in apoptosis, target the nuclear pore or components of the nuclear transport machinery. Four nucleoporins, Nup153, RanBP2, Nup214 and Tpr are cleaved by caspases during apoptosis. In contrast, the nuclear transport factors, Ran, importin alpha and importin beta are not proteolytically processed, but redistribute across the nuclear envelope independently and prior to caspase activation. Also, mRNA accumulates into the nucleus before caspases become active. Microinjection experiments further revealed that early in apoptosis, the nucleus becomes permeable to dextran molecules of 70 kD molecular weight. Redistribution of import factors and mRNA, as well as nuclear permeabilisation, occur prior to caspase-mediated nucleoporin cleavage. Our findings suggest that the apoptotic programme includes modifications in the machinery responsible for nucleocytoplasmic transport, which are independent from caspase-mediated degradation of nuclear proteins.

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Language(s): eng - English
 Dates: 2001-05
 Publication Status: Published in print
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Title: Cell Death and Differentiation
Source Genre: Journal
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Pages: - Volume / Issue: 8 (5) Sequence Number: - Start / End Page: 495 - 505 Identifier: -