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  Spectroscopic methods for the determination of protein interactions

Groemping, Y., & Hellmann, N. (2005). Spectroscopic methods for the determination of protein interactions. In Current Protocols in Protein Science (pp. 1-27). Hoboken (New Jersey): John Wiley & Sons, Inc. doi:10.1002/0471140864.ps2008s39.

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CurrProtProteinSci_39_2005_20.8.1Suppl.pdf (Any fulltext), 311KB
 
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Groemping, Yvonne1, Author              
Hellmann, Nadja, Author
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1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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 Abstract: This unit provides guidelines on how to use steady-state fluorescence spectroscopy for the quantification of protein-protein interactions. The fluorescence of a protein is characterized by its excitation and emission spectra, quantum yield, and anisotropy. These parameters can change upon interaction with another protein and can be used to measure the extent of complex formation. The source of fluorescence can be an intrinsic fluorophore, such as tryptophan or tyrosine; a covalently attached fluorescent dye; or a fluorescent binding partner, such as a nucleotide or cofactor, that interacts specifically with the complex. Protocols are provided in this unit for determining affinity constants and stoichiometry values for protein-protein interactions using equilibrium titration experiments. In addition, fluorescent labeling of proteins is discussed, and an introduction to data analysis is provided. Most of the topics addressed in this unit can easily be applied to other spectroscopic methods or to the analysis of protein-ligand interactions.

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Language(s): eng - English
 Dates: 2005-03-012005-03-01
 Publication Status: Published in print
 Pages: 27
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 Table of Contents: -
 Rev. Type: Peer
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Title: Current Protocols in Protein Science
  Alternative Title : Curr. Protoc. Protein Sc.
Source Genre: Book
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Publ. Info: Hoboken (New Jersey) : John Wiley & Sons, Inc.
Pages: - Volume / Issue: 39 Sequence Number: - Start / End Page: 1 - 27 Identifier: ISBN: 9780471140863