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  A new high sensitivity 19F probe for labeling cysteine groups of proteins

Kalbitzer, H. R., Rohr, G., Nowak, E., Goody, R. S., Kuhn, W., & Zimmermann, H. (1992). A new high sensitivity 19F probe for labeling cysteine groups of proteins. NMR in Biomedicine, 5(6), 347-350. doi:10.1002/nbm.1940050605.

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Genre: Journal Article
Alternative Title : A novel 19F label for NMR spectroscopy and tomography

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NMRBiomed_5_1992_347.pdf (Any fulltext), 336KB
 
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 Creators:
Kalbitzer, Hans Robert1, Author              
Rohr, Gerhard, Author
Nowak, E., Author
Goody, Roger S.1, Author              
Kuhn, W., Author
Zimmermann, Herbert1, 2, 3, Author              
Affiliations:
1Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              
2Department of Molecular Physics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497705              
3Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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 Abstract: A novel iodoacetamide label 4-perfluoro-tert-butyl-phenyliodoacetamide (PFP) containing nine fluorine atoms in equivalent positions has been synthesized. It provides a homogeneous 19F NMR resonance line which can be detected with high sensitivity when coupled to proteins. As an example, the sulfhydryl groups of actin have been labeled with PFP; < 100 nmol of this medium sized protein (corresponding to 2.5 mL of a 40 microM solution) can be detected easily in a single scan at 470 MHz.

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Language(s): eng - English
 Dates: 1992-03-241991-09-271992-03-242005-12-151992-12-01
 Publication Status: Published in print
 Pages: 4
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 Rev. Type: Peer
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Title: NMR in Biomedicine
Source Genre: Journal
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Publ. Info: London : Heyden & Son
Pages: - Volume / Issue: 5 (6) Sequence Number: - Start / End Page: 347 - 350 Identifier: ISSN: 0952-3480
CoNE: https://pure.mpg.de/cone/journals/resource/954925574973