A new high sensitivity 19F probe for labeling cysteine groups of proteins

Kalbitzer, Hans Robert; Rohr, Gerhard; Nowak, E.; Goody, Roger S.; Kuhn, W.; Zimmermann, Herbert

doi:10.1002/nbm.1940050605

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A new high sensitivity 19F probe for labeling cysteine groups of proteins

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Kalbitzer, Hans Robert
Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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Goody, Roger S.
Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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Zimmermann, Herbert
Department of Molecular Physics, Max Planck Institute for Medical Research, Max Planck Society;
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;
Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Kalbitzer, H. R., Rohr, G., Nowak, E., Goody, R. S., Kuhn, W., & Zimmermann, H. (1992). A new high sensitivity 19F probe for labeling cysteine groups of proteins. NMR in Biomedicine, 5(6), 347-350. doi:10.1002/nbm.1940050605.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0019-ABC0-8

Abstract

A novel iodoacetamide label 4-perfluoro-tert-butyl-phenyliodoacetamide (PFP) containing nine fluorine atoms in equivalent positions has been synthesized. It provides a homogeneous 19F NMR resonance line which can be detected with high sensitivity when coupled to proteins. As an example, the sulfhydryl groups of actin have been labeled with PFP; < 100 nmol of this medium sized protein (corresponding to 2.5 mL of a 40 microM solution) can be detected easily in a single scan at 470 MHz.