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  Super-resolution microscopy of mitochondria.

Jakobs, S., & Wurm, C. A. (2014). Super-resolution microscopy of mitochondria. Current Opinion in Chemical Biology, 20, 9-15. doi:10.1016/j.cbpa.2014.03.019.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-8B7B-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-CDB5-6
Genre: Journal Article

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2029419.pdf (Publisher version), 2MB
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 Creators:
Jakobs, S.1, Author              
Wurm, C. A.2, Author              
Affiliations:
1Research Group of Mitochondrial Structure and Dynamics, MPI for biophysical chemistry, Max Planck Society, ou_578566              
2Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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 Abstract: Mitochondria, the powerhouses of the cell, are essential organelles in eukaryotic cells. With their complex inner architecture featuring a smooth outer and a highly convoluted inner membrane, they are challenging objects for microscopy. The diameter of mitochondria is generally close to the resolution limit of conventional light microscopy, rendering diffraction-unlimited super-resolution light microscopy (nanoscopy) for imaging submitochondrial protein distributions often mandatory. In this review, we discuss what can be expected when imaging mitochondria with conventional diffraction-limited and diffraction-unlimited microscopy. We provide an overview on recent studies using super-resolution microscopy to investigate mitochondria and discuss further developments and challenges in mitochondrial biology that might by addressed with these technologies in the future.

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Language(s): eng - English
 Dates: 2014-06
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.cbpa.2014.03.019
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Title: Current Opinion in Chemical Biology
Source Genre: Journal
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Pages: - Volume / Issue: 20 Sequence Number: - Start / End Page: 9 - 15 Identifier: -