English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Fluorescence nanoscopy by polarization modulation and polarization angle narrowing.

Hafi, N., Grunwald, M., van den Heuvel, L. S., Aspelmeier, T., Chen, J., Zagrebelsky, M., et al. (2014). Fluorescence nanoscopy by polarization modulation and polarization angle narrowing. Nature Methods, 11(5), 579-584. doi:10.1038/NMETH.2919.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-B811-0 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-9A91-D
Genre: Journal Article

Files

show Files
hide Files
:
2032678.pdf (Publisher version), 2MB
Name:
2032678.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2032678_Suppl_1.pdf (Supplementary material), 945KB
Name:
2032678_Suppl_1.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2032678_Suppl_2.zip (Supplementary material), 7MB
Name:
2032678_Suppl_2.zip
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/zip / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2032678_Suppl_3.pdf (Supplementary material), 89KB
Name:
2032678_Suppl_3.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
Hafi, N., Author
Grunwald, M.1, Author              
van den Heuvel, L. S., Author
Aspelmeier, T.2, Author              
Chen, J.1, Author              
Zagrebelsky, M., Author
Schütte, O. M., Author
Steinem, C., Author
Korte, M., Author
Munk, A.2, Author              
Walla, P. J.1, Author              
Affiliations:
1Research Group of Biomolecular Spectroscopy and Single-Molecule Detection, MPI for biophysical chemistry, Max Planck Society, ou_578565              
2Research Group of Statistical Inverse-Problems in Biophysics, MPI for Biophysical Chemistry, Max Planck Society, ou_1113580              

Content

show
hide
Free keywords: -
 Abstract: When excited with rotating linear polarized light, differently oriented fluorescent dyes emit periodic signals peaking at different times. We show that measurement of the average orientation of fluorescent dyes attached to rigid sample structures mapped to regularly defined (50 nm)(2) image nanoareas can provide subdiffraction resolution (super resolution by polarization demodulation, SPoD). Because the polarization angle range for effective excitation of an oriented molecule is rather broad and unspecific, we narrowed this range by simultaneous irradiation with a second, de-excitation, beam possessing a polarization perpendicular to the excitation beam (excitation polarization angle narrowing, ExPAN). This shortened the periodic emission flashes, allowing better discrimination between molecules or nanoareas. Our method requires neither the generation of nanometric interference structures nor the use of switchable or blinking fluorescent probes. We applied the method to standard wide-field microscopy with camera detection and to two-photon scanning microscopy, imaging the fine structural details of neuronal spines.

Details

show
hide
Language(s): eng - English
 Dates: 2014-04-062014-05
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1038/NMETH.2919
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nature Methods
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 11 (5) Sequence Number: - Start / End Page: 579 - 584 Identifier: -