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  Staining and embedding the whole mouse brain for electron microscopy

Mikula, S., Binding, J., & Denk, W. (2012). Staining and embedding the whole mouse brain for electron microscopy. Nature methods, 9(12), 1198-1201. doi:10.1038/nmeth.2213.

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Genre: Journal Article
Alternative Title : Staining and embedding the whole mouse brain for electron microscopy

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NatMeth_9_2012_1198.pdf (Any fulltext), 2MB
 
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Mikula, Shawn1, Author              
Binding, Jonas1, Author              
Denk, Winfried1, Author              
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1Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              

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 Abstract: The development of methods for imaging large contiguous volumes with the electron microscope could allow the complete mapping of a whole mouse brain at the single−axon level. We developed a method based on prolonged immersion that enables staining and embedding of the entire mouse brain with uniform myelin staining and a moderate preservation of the tissue's ultrastructure. We tested the ability to follow myelinated axons using serial block−face electron microscopy

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Language(s): eng - English
 Dates: 2012-05-142012-09-282012-10-212012-12-01
 Publication Status: Published in print
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 Rev. Type: Peer
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Title: Nature methods
  Other : Nature methods
Source Genre: Journal
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Publ. Info: New York, NY : Nature Pub. Group
Pages: - Volume / Issue: 9 (12) Sequence Number: - Start / End Page: 1198 - 1201 Identifier: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556