English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Charting Monosynaptic Connectivity Maps by Two-Color Light-Sheet Fluorescence Microscopy

Niedworok, C., Schwarz, I., Ledderose, J., Giese, G., Conzelmann, K., & Schwarz, M. K. (2012). Charting Monosynaptic Connectivity Maps by Two-Color Light-Sheet Fluorescence Microscopy. Cell Reports, 2(5), 1375-1386. doi:10.1016/j.celrep.2012.10.008.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-0852-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-0853-2
Genre: Journal Article
Alternative Title : Charting Monosynaptic Connectivity Maps by Two-Color Light-Sheet Fluorescence Microscopy

Files

show Files
hide Files
:
CellRep_2_2012_1375 .pdf (Any fulltext), 5MB
 
File Permalink:
-
Name:
CellRep_2_2012_1375 .pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
Niedworok, Christian1, 2, Author              
Schwarz, Inna1, Author              
Ledderose, Julia1, Author              
Giese, Günter2, Author              
Conzelmann, Karl−Klaus, Author
Schwarz, Martin K.1, Author              
Affiliations:
1Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              
2Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              

Content

show
hide
Free keywords: -
 Abstract: Cellular resolution three−dimensional (3D) visualization of defined, fluorescently labeled long−range neuronal networks in the uncut adult mouse brain has been elusive. Here, a virus−based strategy is described that allowed fluorescent labeling of centrifugally projecting neuronal populations in the ventral forebrain and their directly, monosynaptically connected bulbar interneurons upon a single stereotaxic injection into select neuronal populations. Implementation of improved tissue clearing combined with light−sheet fluorescence microscopy permitted imaging of the resulting connectivity maps in a single whole−brain scan. Subsequent 3D reconstructions revealed the exact distribution of the diverse neuronal ensembles monosynaptically connected with distinct bulbar interneuron populations. Moreover, rehydratation of brains after light−sheet fluorescence imaging enabled the immunohistochemical identification of synaptically connected neurons. Thus, this study describes a method for identifying monosynaptic connectivity maps from distinct, virally labeled neuronal populations that helps in better understanding of information flow in neural systems

Details

show
hide
Language(s): eng - English
 Dates: 2012-03-052012-10-112012-11-082012-11-29
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Cell Reports
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 2 (5) Sequence Number: - Start / End Page: 1375 - 1386 Identifier: Other: 2211-1247
CoNE: https://pure.mpg.de/cone/journals/resource/2211-1247