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  Microprocessor activity controls differential miRNA biogenesis In Vivo

Conrad, T., Marsico, A., Gehre, M., & Ørom, U. A. (2014). Microprocessor activity controls differential miRNA biogenesis In Vivo. Cell Reports, 9(2), 542-554. doi:10.1016/j.celrep.2014.09.007.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0025-0BA5-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0025-0BA6-9
Genre: Journal Article

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© 2014 Elsevier B.V.
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 Creators:
Conrad, Thomas1, Author              
Marsico, Annalisa2, Author              
Gehre, Maja1, Author              
Ørom, Ulf Andersson1, Author              
Affiliations:
1Long non-coding RNA (Ulf Andersson Ørom), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479659              
2Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433547              

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 Abstract: In miRNA biogenesis, pri-miRNA transcripts are converted into pre-miRNA hairpins. The in vivo properties of this process remain enigmatic. Here, we determine in vivo transcriptome-wide pri-miRNA processing using next-generation sequencing of chromatin-associated pri-miRNAs. We identify a distinctive Microprocessor signature in the transcriptome profile from which efficiency of the endogenous processing event can be accurately quantified. This analysis reveals differential susceptibility to Microprocessor cleavage as a key regulatory step in miRNA biogenesis. Processing is highly variable among pri-miRNAs and a better predictor of miRNA abundance than primary transcription itself. Processing is also largely stable across three cell lines, suggesting a major contribution of sequence determinants. On the basis of differential processing efficiencies, we define functionality for short sequence features adjacent to the pre-miRNA hairpin. In conclusion, we identify Microprocessor as the main hub for diversified miRNA output and suggest a role for uncoupling miRNA biogenesis from host gene expression.

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Language(s): eng - English
 Dates: 2014-10-092014-10-23
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.celrep.2014.09.007
ISSN: 2211-1247 (Electronic)
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Title: Cell Reports
Source Genre: Journal
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Publ. Info: Elsevier
Pages: - Volume / Issue: 9 (2) Sequence Number: - Start / End Page: 542 - 554 Identifier: Other: 2211-1247
CoNE: https://pure.mpg.de/cone/journals/resource/2211-1247