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  Structures of intermediates during RES complex assembly.

Wysoczanski, P., Becker, S., & Zweckstetter, M. (2015). Structures of intermediates during RES complex assembly. Scientific Reports, 5: 12545. doi:10.1038/srep12545.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-1A66-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-2287-0
Genre: Journal Article

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Wysoczanski, P.1, Author              
Becker, S.2, Author              
Zweckstetter, M.1, Author              
Affiliations:
1Research Group of Protein Structure Determination using NMR, MPI for biophysical chemistry, Max Planck Society, ou_578571              
2Department of NMR Based Structural Biology, MPI for biophysical chemistry, Max Planck Society, ou_578567              

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 Abstract: The action of the spliceosome depends on the stepwise cooperative assembly and disassembly of its components. Very strong cooperativity was observed for the RES (Retention and Splicing) hetero-trimeric complex where the affinity from binary to tertiary interactions changes more than 100-fold and affects RNA binding. The RES complex is involved in splicing regulation and retention of not properly spliced pre-mRNA with its three components-Snu17p, Pml1p and Bud13p-giving rise to the two possible intermediate dimeric complexes Pml1p-Snu17p and Bud13p-Snu17p. Here we determined the three-dimensional structure and dynamics of the Pml1p-Snu17p and Bud13p-Snu17p dimers using liquid state NMR. We demonstrate that localized as well as global changes occur along the RES trimer assembly pathway. The stepwise rigidification of the Snu17p structure following the binding of Pml1p and Bud13p provides a basis for the strong cooperative nature of RES complex assembly.

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Language(s): eng - English
 Dates: 2015-07-27
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.1038/srep12545
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Title: Scientific Reports
Source Genre: Journal
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Pages: 10 Volume / Issue: 5 Sequence Number: 12545 Start / End Page: - Identifier: -