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キーワード:
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要旨:
Circular RNAs (circRNAs) are an endogenous class of animal RNAs. Despite
their abundance, their function and expression in the nervous system are
unknown. Therefore, we sequenced RNA from different brain regions,
primary neurons, isolated synapses, as well as during neuronal
differentiation. Using these and other available data, we discovered and
analyzed thousands of neuronal human and mouse circRNAs. circRNAs were
extraordinarily enriched in the mammalian brain, well conserved in
sequence, often expressed as circRNAs in both human and mouse, and
sometimes even detected in Drosophila brains. circRNAs were overall
upregulated during neuronal differentiation, highly enriched in
synapses, and often differentially expressed compared to their mRNA
isoforms. circRNA expression correlated negatively with expression of
the RNA-editing enzyme ADAR1. Knockdown of ADAR1 induced elevated
circRNA expression. Together, we provide a circRNA brain expression
atlas and evidence for important circRNA functions and values as
biomarkers.
