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  Developmental profile of kainate receptor subunit KA1 revealed by Cre expression in YAC transgenic mice

Kask, K., Jerecic, J., Zamanillo, D., Wilbertz, J., Sprengel, R., & Seeburg, P. H. (2000). Developmental profile of kainate receptor subunit KA1 revealed by Cre expression in YAC transgenic mice. Brain Research, 876(1-2), 55-61. doi:10.1016/S0006-8993(00)02599-3.

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Genre: Journal Article
Alternative Title : Developmental profile of kainate receptor subunit KA1 revealed by Cre expression in YAC transgenic mice

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 Creators:
Kask, Kalev, Author
Jerecic, Jasna1, Author              
Zamanillo, Daniel1, Author              
Wilbertz, Johannes2, Author              
Sprengel, Rolf1, Author              
Seeburg, Peter H.1, Author              
Affiliations:
1Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              
2Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              

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Free keywords: High-affinity kainate receptor subunit; YAC transgenic; Cre recombinase; lacZ reporter mice
 Abstract: To determine the spatio-temporal expression in brain of the high-affinity kainate receptor subunit KA1, we generated transgenic mice expressing Cre recombinase from the KA1 gene on a chromosomally integrated 550 kb yeast artificial chromosome (YAC). Activity of the KA1 gene promoter during brain development was visualized by Cre immunohistochemistry, and by X-gal staining of b-galactosidase induced by Cre recombinase in double transgenic KA1-Cre/lacZ indicator mice. During early brain development, expression from the YAC-carried KA1-Cre transgene was observed in all major brain areas, predicting a function for KA1 in the developing central nervous system. In the adult brain, KA1-Cre transgene expression was restricted mainly to hippocampal CA3 pyramidal and dentate gyrus granule cells, an adult expression pattern characteristic for the endogenous KA1 alleles. KA1-Cre transgenic mice may help in elucidating the role of floxed genes ablated in vivo in KA1 expressing neurons

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Language(s): eng - English
 Dates: 2000-06-202000-08-302000-09-08
 Publication Status: Published in print
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Brain Research
  Other : Brain Res.
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 876 (1-2) Sequence Number: - Start / End Page: 55 - 61 Identifier: ISSN: 0006-8993
CoNE: https://pure.mpg.de/cone/journals/resource/954926250616