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  Cytoskeleton remodelling of confluent epithelial cells cultured on porous substrates

Rother, J., Buchsenschutz-Gobeler, M., Noding, H., Steltenkamp, S., Samwer, K., & Janshoff, A. (2015). Cytoskeleton remodelling of confluent epithelial cells cultured on porous substrates. Journal of the Royal Society, Interface / the Royal Society, 12(103): 20141057. doi:10.1098/rsif.2014.1057.

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http://www.ncbi.nlm.nih.gov/pubmed/25566882 (beliebiger Volltext)
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Rother, J., Autor
Buchsenschutz-Gobeler, M., Autor
Noding, H., Autor
Steltenkamp, S.1, Autor           
Samwer, K., Autor
Janshoff, A., Autor
Affiliations:
1Micro Systems Technology, Center of Advanced European Studies and Research (caesar), Max Planck Society, ou_2173685              

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 Zusammenfassung: The impact of substrate topography on the morphological and mechanical properties of confluent MDCK-II cells cultured on porous substrates was scrutinized by means of various imaging techniques as well as atomic force microscopy comprising force volume and microrheology measurements. Regardless of the pore size, ranging from 450 to 5500 nm in diameter, cells were able to span the pores. They did not crawl into the holes or grow around the pores. Generally, we found that cells cultured on non-porous surfaces are stiffer, i.e. cortical tension rises from 0.1 to 0.3 mN m(-1), and less fluid than cells grown over pores. The mechanical data are corroborated by electron microscopy imaging showing more cytoskeletal filaments on flat samples in comparison to porous ones. By contrast, cellular compliance increases with pore size and cells display a more fluid-like behaviour on larger pores. Interestingly, cells on pores larger than 3500 nm produce thick actin bundles that bridge the pores and thereby strengthen the contact zone of the cells.

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 Datum: 2015
 Publikationsstatus: Erschienen
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 Ort, Verlag, Ausgabe: -
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 Identifikatoren: Anderer: 25566882
DOI: 10.1098/rsif.2014.1057
ISSN: 1742-5662 (Electronic)
ISSN: 1742-5662 (Linking)
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Titel: Journal of the Royal Society, Interface / the Royal Society
  Alternativer Titel : J. R. Soc. Interface
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 12 (103) Artikelnummer: 20141057 Start- / Endseite: - Identifikator: -