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  Volta phase plate cryo-EM of the small protein complex Prx3

Khoshouei, M., Radjainia, M., Phillips, A. J., Gerrard, J. A., Mitra, A. K., Plitzko, J. M., et al. (2016). Volta phase plate cryo-EM of the small protein complex Prx3. NATURE COMMUNICATIONS, 7: 10534. doi:10.1038/ncomms10534.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-C7F9-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-C7FA-9
Genre: Journal Article

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 Creators:
Khoshouei, Maryam1, Author              
Radjainia, Mazdak2, Author
Phillips, Amy J.2, Author
Gerrard, Juliet A.2, Author
Mitra, Alok K.2, Author
Plitzko, Jürgen M.1, Author              
Baumeister, Wolfgang1, Author              
Danev, Radostin1, Author              
Affiliations:
1Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              
2external, ou_persistent22              

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Free keywords: CRYOELECTRON MICROSCOPY; ELECTRON-MICROSCOPY; ANGSTROM RESOLUTION; PEROXIREDOXIN; CRYOMICROSCOPY; TOMOGRAPHY
 Abstract: Cryo-EM of large, macromolecular assemblies has seen a significant increase in the numbers of high-resolution structures since the arrival of direct electron detectors. However, sub-nanometre resolution cryo-EM structures are rare compared with crystal structure depositions, particularly for relatively small particles (<400 kDa). Here we demonstrate the benefits of Volta phase plates for single-particle analysis by time-efficient cryo-EM structure determination of 257 kDa human peroxiredoxin-3 dodecamers at 4.4 angstrom resolution. The Volta phase plate improves the applicability of cryo-EM for small molecules and accelerates structure determination.

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Language(s): eng - English
 Dates: 2016
 Publication Status: Published online
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: ISI: 000369024800001
DOI: 10.1038/ncomms10534
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Title: NATURE COMMUNICATIONS
Source Genre: Journal
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Publ. Info: MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND : NATURE PUBLISHING GROUP
Pages: - Volume / Issue: 7 Sequence Number: 10534 Start / End Page: - Identifier: ISSN: 2041-1723