English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Hedgehog signaling strength is orchestrated by the mir-310 cluster of microRNAs in response to diet.

Cicek, I. Ö., Karaca, S., Brankatschk, M., Eaton, S., Urlaub, H., & Shcherbata, H. R. (2016). Hedgehog signaling strength is orchestrated by the mir-310 cluster of microRNAs in response to diet. Genetics, 202(3), 1167-1183. doi:10.1534/genetics.115.185371.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-040C-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-A028-1
Genre: Journal Article

Files

show Files
hide Files
:
2256412.pdf (Publisher version), 5MB
 
File Permalink:
-
Name:
2256412.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Biophysical Chemistry (Karl Friedrich Bonhoeffer Institute), Göttingen; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2256412_Suppl.pdf (Supplementary material), 2MB
Name:
2256412_Suppl.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
Cicek, I. Ö.1, Author              
Karaca, S.2, Author              
Brankatschk, M., Author
Eaton, S., Author
Urlaub, H.2, Author              
Shcherbata, H. R.1, Author              
Affiliations:
1Research Group of Gene Expression and Signaling, MPI for biophysical chemistry, Max Planck Society, ou_578608              
2Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society, ou_578613              

Content

show
hide
Free keywords: Drosophila; Hedgehog signaling; follicle stem cell; miRNA; oogenesis
 Abstract: Since the discovery of miRNAs only two decades ago, they have emerged as an essential component of the gene regulatory machinery. miRNAs have seemingly paradoxical features: a single miRNA is able to simultaneously target hundreds of genes, while its presence is mostly dispensable for animal viability under normal conditions. It is known that miRNAs act as stress response factors; however, it remains challenging to determine their relevant targets and the conditions under which they function. To address this challenge, we propose a new workflow for miRNA function analysis, using which we found that the evolutionarily young miRNA family, the mir-310s, are important regulators of Drosophila metabolic status. mir-310s-deficient animals have an abnormal diet-dependent expression profile for numerous diet-sensitive components, accumulate fats, and show various physiological defects. We found that the mir-310s simultaneously repress the production of several regulatory factors (Rab23, DHR96 and Ttk) of the evolutionarily conserved Hedgehog (Hh) pathway to sharpen dietary response. As the mir-310s expression is highly dynamic and nutrition-sensitive, this signal relay model helps to explain the molecular mechanism governing quick and robust Hh signaling responses to nutritional changes. Additionally, we discovered a new component of the Hh signaling pathway in Drosophila, Rab23, which cell autonomously regulates Hh ligand trafficking in the germline stem cell niche. How organisms adjust to dietary fluctuations to sustain healthy homeostasis is an intriguing research topic. These data are the first report showing that miRNAs can act as executives that transduce nutritional signals to an essential signaling pathway. This suggests miRNAs as plausible therapeutic agents that can be used in combination with low calorie and cholesterol diets to manage quick and precise tissue-specific responses to nutritional changes.

Details

show
hide
Language(s): eng - English
 Dates: 2016-01-22
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1534/genetics.115.185371
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Genetics
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 202 (3) Sequence Number: - Start / End Page: 1167 - 1183 Identifier: -