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  Quantitative analysis of the Escherichia coli proteome.

Wisniewski, J. R., & Rakus, D. (2014). Quantitative analysis of the Escherichia coli proteome. Data in brief, 1, 7-11. doi:10.1016/j.dib.2014.08.004.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-002A-3AD0-7 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-002A-3AD6-C
Genre: Journal Article

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1-s2.0-S2352340914000079-main.pdf (Any fulltext), 536KB
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 Creators:
Wisniewski, Jacek R.1, Author           
Rakus, Dariusz2, Author
Affiliations:
1Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              
2external, ou_persistent22              

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Free keywords: Absolute protein quantification; Escherichia coli proteome; Filter-aided sample preparation, FASP; Protein copy number; Total protein approach
 Abstract: Escherichia coli (strain ATCC 25922 in a stationary culture) cells were lysed with SDS and the lysates were processed according MED-FASP protocol. The released peptides were analyzed by LC-MS/MS. Protein content per bacterial cell was calculated on the basis of the DNA content. Absolute protein quantitation was performed using the 'Total Protein Approach'. The data are supplied in the article.

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Language(s): eng - English
 Dates: 2014
 Publication Status: Published online
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 26217677
DOI: 10.1016/j.dib.2014.08.004
 Degree: -

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Title: Data in brief
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 1 Sequence Number: - Start / End Page: 7 - 11 Identifier: ISSN: 2352-3409