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  Multifaceted biological insights from a draft genome sequence of the tobacco hornworm moth, Manduca sexta

Kanost, M. R., Arrese, E. L., Cao, X., Chen, Y.-R., Chellapilla, S., Goldsmith, M., et al. (2016). Multifaceted biological insights from a draft genome sequence of the tobacco hornworm moth, Manduca sexta. Insect Biochemistry and Molecular Biology, 76, 118-147. doi:10.1016/j.ibmb.2016.07.005.

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 Creators:
Kanost, Michael R., Author
Arrese, Estela L., Author
Cao, Xiaolong, Author
Chen, Yun-Ru, Author
Chellapilla, Sanjay, Author
Goldsmith, Marian, Author
Grosse-Wilde, Ewald1, Author              
Heckel, David G.2, Author              
Herndon, Nicolae, Author
Jiang, Haobo, Author
Papanicolaou, Alexie, Author
Qu, Jiaxin, Author
Soulages, Jose L., Author
Vogel, Heiko2, Author              
Walters, James, Author
Waterhouse, Robert M., Author
Ahn, Seung-Joon2, Author              
Almeida, Francisca C., Author
An, Chunju, Author
Aqrawi, Peshtewani, Author
Bretschneider, Anne2, 3, Author              Bryant, William B., AuthorBucks, Sascha1, Author              Chao, Hsu, AuthorChevignon, Germain, AuthorChristen, Jayne M., AuthorClarke, David F., AuthorDittmer, Neal T., AuthorFerguson, Laura C.F., AuthorGaravelou, Spyridoula, AuthorGordon, Karl H.J., AuthorGunaratna, Ramesh T., AuthorHan, Yi, AuthorHauser, Frank, AuthorHe, Yan, AuthorHeidel-Fischer, Hanna M.2, Author              Hirsh, Ariana, AuthorHu, Yingxia, AuthorJiang, Hongbo, AuthorKalra, Divya, AuthorKlinner, Christian1, Author              König, Christopher1, 3, Author              Kovar, Christie, AuthorKroll, Ashley R., AuthorKuwar, Suyog2, 3, Author              Lee, Sandy L., AuthorLehman, Rüdiger, AuthorLi, Kai, AuthorLi, Zhaofei, AuthorLiang, Hanquan, AuthorLovelace, Shanna, AuthorLu, Zhiqiang, AuthorMansfield, Jennifer H., AuthorMcCulloch, Kyle J., AuthorMathew, Tittu, AuthorMorton, Brian, AuthorMuzny, Donna M., AuthorNeunemann, David, AuthorOngeri, Fiona, AuthorPauchet, Yannick2, Author              Pu, Ling-Ling, AuthorPyrousis, Ioannis, AuthorRao, Xiang-Jun, AuthorRedding, Amanda, AuthorRoesel, Charles, AuthorSanchez-Gracia, Alejandro, AuthorSchaack, Sarah, AuthorShukla, Aditi, AuthorTetreau, Guillaume, AuthorWang, Yang, AuthorXiong, Guang-Hua, AuthorTraut, Walther, AuthorWalsh, Tom K., AuthorWorley, Kim C., AuthorWu, Di, AuthorWu, Wenbi, AuthorWu, Yuan-Qing, AuthorZhang, Xiufeng, AuthorZou, Zhen, AuthorZucker, Hannah, AuthorBriscoe, Adriana D., AuthorBurmester, Thorsten, AuthorClem, Rollie, AuthorFeyereisen, René, AuthorGrimmelikhuijzen, Cornelis J.P., AuthorHamodrakas, Stavros J., AuthorHansson, Bill S.1, Author              Huguet, Elisabeth, AuthorJermiin, Lars S., AuthorLan, Que, AuthorLehman, Herman K., AuthorLorenzen, Marce, AuthorMerzendorfer, Hans, AuthorMichalopoulos, Ioannis, AuthorMorton, David B., AuthorMuthukrishnan, Subbaratnam, AuthorOakeshott, John G., AuthorPalmer, Will, AuthorPark, Yoonseong, AuthorPassarelli, A. Lorena, AuthorRozas, Julio, AuthorSchwartz, Lawrence M., AuthorSmith, Wendy, AuthorSouthgate, Agnes, AuthorVilcinskas, Andreas, AuthorVogt, Richard, AuthorWang, Ping, AuthorWerren, John, AuthorYu, Xiao-Qiang, AuthorZhou, Jing-Jiang, AuthorBrown, Susan J., AuthorScherer, Steven E., AuthorRichards, Stephen, AuthorBlissard, Gary W., Author more..
Affiliations:
1Department of Evolutionary Neuroethology, Prof. B. S. Hansson, MPI for Chemical Ecology, Max Planck Society, ou_421894              
2Department of Entomology, Prof. D. G. Heckel, MPI for Chemical Ecology, Max Planck Society, ou_421895              
3IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society, Jena, DE, ou_421900              

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 Abstract: Manduca sexta, known as the tobacco hornworm or Carolina sphinx moth, is a lepidopteran insect that is used extensively as a model system for research in insect biochemistry, physiology, neurobiology, development, and immunity. One important benefit of this species as an experimental model is its extremely large size, reaching more than 10 g in the larval stage. M. sexta larvae feed on solanaceous plants and thus must tolerate a substantial challenge from plant allelochemicals, including nicotine. We report the sequence and annotation of the M. sexta genome, and a survey of gene expression in various tissues and developmental stages. The Msex_1.0 genome assembly resulted in a total genome size of 419.4 Mbp. Repetitive sequences accounted for 25.8% of the assembled genome. The official gene set is comprised of 15,451 protein-coding genes, of which 2498 were manually curated. Extensive RNA-seq data from many tissues and developmental stages were used to improve gene models and for insights into gene expression patterns. Genome wide synteny analysis indicated a high level of macrosynteny in the Lepidoptera. Annotation and analyses were carried out for gene families involved in a wide spectrum of biological processes, including apoptosis, vacuole sorting, growth and development, structures of exoskeleton, egg shells, and muscle, vision, chemosensation, ion channels, signal transduction, neuropeptide signaling, neurotransmitter synthesis and transport, nicotine tolerance, lipid metabolism, and immunity. This genome sequence, annotation, and analysis provide an important new resource from a well-studied model insect species and will facilitate further biochemical and mechanistic experimental studies of many biological systems in insects.

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 Dates: 2016-07-142016-08-122016
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: HEC342
DOI: 10.1016/j.ibmb.2016.07.005
 Degree: -

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Title: Insect Biochemistry and Molecular Biology
  Other : Insect Biochem. Mol. Biol.
Source Genre: Journal
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Publ. Info: Oxford [England] : Pergamon
Pages: - Volume / Issue: 76 Sequence Number: - Start / End Page: 118 - 147 Identifier: ISSN: 0965-1748
CoNE: https://pure.mpg.de/cone/journals/resource/954925581163