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  LOVTRAP: an optogenetic system for photoinduced protein dissociation

Wang, H., Vilela, M., Winkler, A., Tarnawski, M., Schlichting, I., Yumerefendi, H., et al. (2016). LOVTRAP: an optogenetic system for photoinduced protein dissociation. Nature methods, 13(9), 755-758. doi:10.1038/nmeth.3926.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-002B-1FDA-D Version Permalink: https://hdl.handle.net/11858/00-001M-0000-002B-9CC7-9
Genre: Journal Article

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 Creators:
Wang, Hui, Author
Vilela, Marco, Author
Winkler, Andreas1, Author           
Tarnawski, Miroslaw1, Author           
Schlichting, Ilme1, Author           
Yumerefendi, Hayretin, Author
Kuhlman, Brian, Author
Liu, Rihe, Author
Danuser, Gaudenz, Author
Hahn, Klaus M, Author
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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Free keywords: Cell migration; Cell signalling; Optogenetics
 Abstract: LOVTRAP is an optogenetic approach for reversible light-induced protein dissociation using protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in the dissociation constant (Kd). By reversibly sequestering proteins at mitochondria, we precisely modulated the proteins' access to the cell edge, demonstrating a naturally occurring 3-mHz cell-edge oscillation driven by interactions of Vav2, Rac1, and PI3K proteins.

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Language(s): eng - English
 Dates: 2016-06-162015-11-212016-07-182016-09-01
 Publication Status: Issued
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/nmeth.3926
URI: http://www.ncbi.nlm.nih.gov/pubmed/27427858
URI: http://www.nature.com/nmeth/journal/vaop/ncurrent/full/nmeth.3926.html?WT.feed_name=subjects_optogenetics
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Title: Nature methods
  Other : Nature methods
Source Genre: Journal
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Publ. Info: New York, NY : Nature Pub. Group
Pages: - Volume / Issue: 13 (9) Sequence Number: - Start / End Page: 755 - 758 Identifier: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556