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  A look at membrane patches with a scanning force microscope

Hörber, J. K. H., Mosbacher, J., Häberle, W., Ruppersberg, J. P., & Sakmann, B. (1995). A look at membrane patches with a scanning force microscope. Biophysical Journal, 68(5), 1687-1693. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/7612812.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-002B-5658-4 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-002B-5659-2
Genre: Journal Article
Alternative Title : A look at membrane patches with a scanning force microscope

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BiophysJ_68_1995_1687.pdf (Any fulltext), 3MB
 
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Hörber, J. K. Heinrich, Author
Mosbacher, Johannes1, Author           
Häberle, Walter, Author
Ruppersberg, J. Peter1, Author           
Sakmann, Bert1, Author           
Affiliations:
1Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              

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 Abstract: We combined scanning force microscopy with patch-clamp techniques in the same experimental setup and obtained images of excised membrane patches spanning the tip of a glass pipette. These images indicate that cytoskeleton structures are still present in such membrane patches and form a strong connection between the membrane and the glass wall. This gives the membrane patch the appearance of a tent, stabilized by a scaffold of ropes. The lateral resolution of the images depends strongly on the observed structures and can reach values as low as 10 nm on the cytoskeleton elements of a (inside-out) patch. The observations suggest that measurements of membrane elasticity can be made, opening the way for further studies on mechanical properties of cell membranes.

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Language(s): eng - English
 Dates: 1994-11-291995-02-011995-05-01
 Publication Status: Issued
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 666734
URI: http://www.ncbi.nlm.nih.gov/pubmed/7612812
URI: http://www.sciencedirect.com/science/article/pii/S0006349595803462
Other: 4154
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Title: Biophysical Journal
  Other : Biophys. J.
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 68 (5) Sequence Number: - Start / End Page: 1687 - 1693 Identifier: Other: 0006-3495
CoNE: https://pure.mpg.de/cone/journals/resource/954925385117