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  Microfluidics-based single cell analysis reveals drug-dependent motility changes in trypanosomes

Hochstetter, A., Stellamanns, E., Deshpande, S., Uppaluri, S., Engstler, M., & Pfohl, T. (2015). Microfluidics-based single cell analysis reveals drug-dependent motility changes in trypanosomes. Lab on a Chip, 15(8), 1961-1968. doi: 10.1039/c5lc00124b.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-79FD-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-D21C-1
Genre: Journal Article

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 Creators:
Hochstetter, Axel, Author
Stellamanns, Eric1, Author              
Deshpande, Siddhart, Author
Uppaluri, Sravanti1, Author              
Engstler, Markus, Author
Pfohl, Thomas1, Author              
Affiliations:
1Group Dynamics of biological matter, Department of Dynamics of Complex Fluids, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society, ou_2063313              

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 Abstract: We present a single cell viability assay, based on chemical gradient microfluidics in combination with optical micromanipulation. Here, we used this combination to in situ monitor the effects of drugs and chemicals on the motility of the flagellated unicellular parasite Trypanosoma brucei; specifically, the local cell velocity and the mean squared displacement (MSD) of the cell trajectories. With our method, we are able to record in situ cell fixation by glutaraldehyde, and to quantify the critical concentration of 2-deoxy-D-glucose required to completely paralyze trypanosomes. In addition, we detected and quantified the impact on cell propulsion and energy generation at much lower 2-deoxy-D-glucose concentrations. Our microfluidics-based approach advances fast cell-based drug testing in a way that allows us to distinguish cytocidal from cytostatic drug effects, screen effective dosages, and investigate the impact on cell motility of drugs and chemicals. Using suramin, we could reveal the impact of the widely used drug on trypanosomes: suramin lowers trypanosome motility and induces cell-lysis after endocytosis.

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Language(s): eng - English
 Dates: 2015-03-102015
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1039/c5lc00124b
 Degree: -

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Title: Lab on a Chip
Source Genre: Journal
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Publ. Info: Royal Society of Chemistry
Pages: - Volume / Issue: 15 (8) Sequence Number: - Start / End Page: 1961 - 1968 Identifier: -