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  Fluorescent Arc/Arg3.1 indicator mice: a versatile tool to study brain activity changes in vitro and in vivo

Grinevich, V., Kolleker, A., Eliava, M., Takada, N., Takuma, H., Fukuzawa, Y., et al. (2009). Fluorescent Arc/Arg3.1 indicator mice: a versatile tool to study brain activity changes in vitro and in vivo. Journal of Neuroscience Methods, 184(1), 25-26. doi:10.1016/j.jneumeth.2009.07.015.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-110B-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-110C-1
Genre: Journal Article
Alternative Title : Fluorescent Arc/Arg3.1 indicator mice: a versatile tool to study brain activity changes in vitro and in vivo

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JNeurosciMeth_184_2009_25.pdf (Any fulltext), 4MB
 
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 Creators:
Grinevich, Valery1, Author              
Kolleker, Aleksandre1, Author              
Eliava, Marina1, Author              
Takada, Naoki, Author
Takuma, Hiroshi1, Author              
Fukuzawa, Y., Author
Shigemoto, Ryuichi, Author
Kuhl, Dietmar, Author
Waters, David Jack2, Author              
Seeburg, Peter H.1, Author              
Osten, Pavel1, Author              
Affiliations:
1Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              
2Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              

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Free keywords: Immediate early genes Arc Arg3.1 Green fluorescent protein (GFP) Transgenic mice Synaptic plasticity Neural circuits Stress
 Abstract: The brain-specific immediate early gene Arc/Arg3.1 is induced in response to a variety of stimuli, including sensory and behavior-linked neural activity. Here we report the generation of transgenic mice, termed TgArc/Arg3.1-d4EGFP, expressing a 4-h half-life form of enhanced green fluorescent protein (d4EGFP) under the control of the Arc/Arg3.1 promoter. We show that d4EGFP-mediated fluorescence faithfully reports Arc/Arg3.1 induction in response to physiological, pathological and pharmacological stimuli, and that this fluorescence permits electrical recording from activated neurons in the live mouse. Moreover, the fluorescent Arc/Arg3.1 indicator revealed activity changes in circumscribed brain areas in distinct modes of stress and in a mouse model of Alzheimer's disease. These findings identify the TgArc/Arg3.1-d4EGFP mouse as a versatile tool to monitor Arc/Arg3.1 induction in neural circuits, both in vitro and in vivo.

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Language(s): eng - English
 Dates: 2009-07-082009-05-222009-07-092009-07-212009-10-30
 Publication Status: Published in print
 Pages: 2
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

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Title: Journal of Neuroscience Methods
  Other : J. Neurosci. Meth.
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 184 (1) Sequence Number: - Start / End Page: 25 - 26 Identifier: ISSN: 0165-0270
CoNE: https://pure.mpg.de/cone/journals/resource/954925480594