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  Early keratinocyte differentiation on micropillar interfaces

Steinberg, T., Schulz, S., Spatz, J. P., Grabe, N., Mussig, E., Kohl, A., et al. (2007). Early keratinocyte differentiation on micropillar interfaces. Nano Letters, 7(2), 287-294. doi:10.1021/nl062271z.

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 Creators:
Steinberg, Thorsten, Author
Schulz, Simon1, Author              
Spatz, Joachim P.1, 2, Author              
Grabe, Niels, Author
Mussig, Eva, Author
Kohl, Annette, Author
Komposch, Gerda, Author
Tomakidi, Pascal, Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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 Abstract: We employed topographical patterning to analyze early keratinocyte differentiation on top of microfabricated pillar arrays. Fibronectin immobilized on pillar "heads" yielded a nucleus-associated granular keratin 1 (K1) pattern in immortalized human gingival keratinocytes (IHGK) at pillar interspaces of 14 mum. Decreasing distances of 11and 8 mum revealed cytoplasmic extension of the early differentiation marker K1 on poly(dimethylsiloxane) (PDMS) pillars. The most extensive cytoplasmic K1 protein distribution noted at the smallest pillar scale coincided with higher ratios of K1 mRNA gene transcription. These experiments suggest that early keratinocyte differentiation was governed by the topographical characteristics of the pillar pattern. Moreover, they form the basis to study cell functions such as differentiation in a defined topologically structured environment.

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Language(s): eng - English
 Dates: 2006-12-072006-09-272006-12-222007-02-01
 Publication Status: Published in print
 Pages: 8
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 Table of Contents: -
 Rev. Type: Peer
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Title: Nano Letters
  Abbreviation : Nano Lett.
Source Genre: Journal
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Publ. Info: Washington, DC : American Chemical Society
Pages: - Volume / Issue: 7 (2) Sequence Number: - Start / End Page: 287 - 294 Identifier: ISSN: 1530-6984
CoNE: https://pure.mpg.de/cone/journals/resource/110978984570403