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  Accurate delivery of single biomolecules by polyethylene glycol coated submicrometer pipettes

Park, C.-W., Knemeyer, J.-P., Marmé, N., Möller, M., Spatz, J. P., Wolfrum, J., et al. (2004). Accurate delivery of single biomolecules by polyethylene glycol coated submicrometer pipettes. Chemical Physics, 301(1), 105-110. doi:10.1016/j.chemphys.2004.03.006.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0028-34B1-1 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-002E-7DF5-2
Genre: Journal Article

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ChemPhys_301_2004_105.pdf (Any fulltext), 313KB
 
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 Creators:
Park, Chong-Woo, Author
Knemeyer, Jens-Peter, Author
Marmé, Nicole, Author
Möller, Martin, Author
Spatz, Joachim P.1, 2, Author           
Wolfrum, Jürgen, Author
Sauer, Markus, Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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 Abstract: There is a great interest to quantitatively manipulate, separate, and deliver low concentrations or even single biomolecules in submicrometer-sized channels. We report the use of cross-linked four-star polyethylene glycol (PEG) as a new coating technique for microchannels. PEG-coating efficiently reduces the electroosmotic flow (EOF) and non-specific adsorption to the glass walls. Our results demonstrate that individual fluorescently labeled hydrophobic cell adhesion proteins (fibronectin molecules) can be drawn through PEG-coated submicrometer pipettes in aqueous solution by electrokinetic forces without the addition of detergents or other additives which potentially deteriorate the activity and specificity of biomolecules.

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Language(s): eng - English
 Dates: 2003-12-302004-03-052004-04-092004-05-31
 Publication Status: Issued
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1016/j.chemphys.2004.03.006
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Title: Chemical Physics
  Other : Chem. Phys.
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier B.V.
Pages: - Volume / Issue: 301 (1) Sequence Number: - Start / End Page: 105 - 110 Identifier: ISSN: 0301-0104
CoNE: https://pure.mpg.de/cone/journals/resource/954925509371