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  Suche nach einem Metaboliten bei Vergiftung mit Desmethylphalloin (DMP)

Puchinger, H., & Wieland, T. (1969). Suche nach einem Metaboliten bei Vergiftung mit Desmethylphalloin (DMP). European Journal of Biochemistry, 11(1), 1-6. doi:10.1111/j.1432-1033.1969.tb00731.x.

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EurJBiochem_11_1969_1.pdf (Any fulltext), 568KB
 
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Puchinger, Herwig1, Author           
Wieland, Theodor1, Author           
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1Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              

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 Abstract: Various observations cited at the beginning of this paper and in the Discussion suggested that phalloidine, a poisonous constituent of the green mushroom Amanita phalloides, would not be toxic by itself, but would be rendered toxic previously in the liver. Using a tritiated toxic derivative of phalloidine ([3H]desmethylphalloin) we found that: (a) in rats, 60% of the administered substance are excreted unchanged in the urine within the first day. A second radioactive substance of low concentration, moving more slowly in paper chromatography, turned out as a product of the self-degradation of [3H]desmethylphalloin; (b) respectively 98% and 99,9% of the radioactivity were extracted with methanol from homogenates of livers of rats or mice which had received [3H]desmethylphalloin 1 to 2 hours before. Aqueous homogenates of liver of poisened rats also contained radioactivity firmly bound to a high molecular weight substance, as shown by gel chromatography. The radioactive substance was totally removed from the carrier, presumably ribosomal material, by adding methanol, and was identified as [3H]desmethylphalloin; (c) [3H]desmethylphalloin was not metabolized on incubation with oxygen and rat liver ribosomes in a NADPH-regenerating system. A radioactive substance extracted from liver microsomes of a poisoned rat also proved to be original toxin. As a result of these experiments it can be concluded that [3H]desmethylphalloin and most probably also phalloidine are not metabolized in the livers of rats and mice.

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Language(s): eng - English
 Dates: 1969-06-021969-07-172005-03-031969-11
 Publication Status: Issued
 Pages: 6
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 Rev. Type: Peer
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Title: European Journal of Biochemistry
Source Genre: Journal
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Publ. Info: Berlin : Published by Springer-Verlag on behalf of the Federation of European Biochemical Societies
Pages: - Volume / Issue: 11 (1) Sequence Number: - Start / End Page: 1 - 6 Identifier: ISSN: 0014-2956
CoNE: https://pure.mpg.de/cone/journals/resource/111097776606040