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  Revision and reannotation of the Halomonas elongata DSM 2581(T) genome.

Pfeiffer, F., Bagyan, I., Alfaro-Espinoza, G., Zamora-Lagos, M.-A., Habermann, B., Marin-Sanguino, A., et al. (2017). Revision and reannotation of the Halomonas elongata DSM 2581(T) genome. MicrobiologyOpen, e465. doi:10.1002/mbo3.465.

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Pfeiffer_mbo3465.pdf (Publisher version), 315KB
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© 2017 The Authors. This is an open access article under the terms of the Creative Commons Attribution License.
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 Creators:
Pfeiffer, Friedhelm1, Author              
Bagyan, Irina2, Author
Alfaro-Espinoza, Gabriela2, Author
Zamora-Lagos, Maria-A.1, Author              
Habermann, Bianca1, Author              
Marin-Sanguino, Alberto2, Author
Oesterhelt, Dieter3, Author              
Kunte, Hans J2, Author
Affiliations:
1Habermann, Bianca / Computational Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1832284              
2external, ou_persistent22              
3Oesterhelt, Dieter / Membrane Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565164              

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Free keywords: Halomonas elongata; frameshift; genome annotation; genome sequencing; halophilic bacteria; sequence revision
 Abstract: The genome of the Halomonas elongata type strain DSM 2581, an industrial producer, was reevaluated using the Illumina HiSeq2500 technology. To resolve duplication-associated ambiguities, PCR products were generated and sequenced. Outside of duplications, 72 sequence corrections were required, of which 24 were point mutations and 48 were indels of one or few bases. Most of these were associated with polynucleotide stretches (poly-T stretch overestimated in 19 cases, poly-C underestimated in 15 cases). These problems may be attributed to using 454 technology for original genome sequencing. On average, the original genome sequence had only one error in 56kb. There were 23 frameshift error corrections in the 29 protein-coding genes affected by sequence revision. The genome has been subjected to major reannotation in order to substantially increase the annotation quality. 2017 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

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Language(s): eng - English
 Dates: 2017-08-11
 Publication Status: Published online
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 Identifiers: ISI: 28349658
DOI: 10.1002/mbo3.465
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Title: MicrobiologyOpen
Source Genre: Journal
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Pages: - Volume / Issue: - Sequence Number: e465 Start / End Page: - Identifier: ISSN: 2045-8827