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  Cell-permeant large stokes shift dyes for transfection-free multicolor nanoscopy.

Butkevich, A., Lukinavicius, G., D'Este, E., & Hell, S. W. (2017). Cell-permeant large stokes shift dyes for transfection-free multicolor nanoscopy. Journal of the American Chemical Society, 139(36), 12378-12381. doi:10.1021/jacs.7b06412.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-E252-5 Version Permalink: http://hdl.handle.net/21.11116/0000-0001-A4E5-3
Genre: Journal Article

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 Creators:
Butkevich, A.1, Author              
Lukinavicius, G.2, Author              
D'Este, E.1, Author              
Hell, S. W.1, Author              
Affiliations:
1Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society, ou_578627              
2Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society, ou_2616691              

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 Abstract: We designed cell-permeant red-emitting fluorescent dye labels with >140 nm Stokes shifts based on 9-iminoanthrone, 9-imino-10-silaxanthone, and 9-imino-10-germaxanthone fluorophores. The corresponding probes selectively targeting mitochondria, lysosomes, and F-actin demonstrate low toxicity and enable stimulated emission depletion (STED) nanoscopy in neurons, human fibroblasts, U2OS, and HeLa cells. In combination with known small Stokes shift dyes, our probes allow live-cell three-color STED nanoscopy of endogenous targets on popular setups with 775 nm STED wavelength.

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Language(s): eng - English
 Dates: 2017-08-282017-09-13
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1021/jacs.7b06412
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Title: Journal of the American Chemical Society
Source Genre: Journal
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Pages: - Volume / Issue: 139 (36) Sequence Number: - Start / End Page: 12378 - 12381 Identifier: -