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  Luciferases with tunable emission wavelengths

Hiblot, J., Yu, Q., Sabbadini, M. D., Reymond, L., Xue, L., Schena, A., et al. (2017). Luciferases with tunable emission wavelengths. Angewandte Chemie, International Edition in English, 56(46), 14556-14560. doi:10.1002/anie.201708277.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-002E-2D2D-A Version Permalink: https://hdl.handle.net/21.11116/0000-0001-479C-0
Genre: Journal Article

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 Creators:
Hiblot, Julien1, Author           
Yu, Qiuliyang1, Author           
Sabbadini , Marina D.B., Author
Reymond, Luc2, Author           
Xue, Lin1, Author           
Schena , Alberto, Author
Sallin , Olivier, Author
Hill , Nicholas, Author
Griss , Rudolf, Author
Johnsson, Kai1, Author           
Affiliations:
1Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society, ou_2364732              
2Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              

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Free keywords: bioluminescent imaging; luciferase; self-labeling protein; sensors; therapeutic drug monitoring
 Abstract: We introduce luciferases whose emission maxima can be tuned to different wavelengths by chemical labeling. The luciferases are chimeras of NanoLuc with either SNAP-tag or HaloTag7. Labeling of the self-labeling tag with a fluorophore shifts the emission maximum of NanoLuc to that of the fluorophore. Luciferases with tunable colors have applications as reporter genes, for the construction of biosensors and in bioimaging.

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Language(s): eng - English
 Dates: 2017-08-112017-09-222017-10-092017-11-13
 Publication Status: Issued
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1002/anie.201708277
URI: https://www.ncbi.nlm.nih.gov/pubmed/28941028
 Degree: -

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Title: Angewandte Chemie, International Edition in English
  Other : Angewandte Chemie International Edition in English
  Other : Angew. Chem., Int. Ed. Engl.
  Other : Angew. Chem. Int. Ed. Engl.
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: 56 (46) Sequence Number: - Start / End Page: 14556 - 14560 Identifier: ISSN: 0570-0833
CoNE: https://pure.mpg.de/cone/journals/resource/0570-0833