English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
 DetailsSummary
  Automated glycan assembly of galactosylated xyloglucan oligosaccharides and their recognition by plant cell wall glycan-directed antibodies

Dallabernardina, P., Ruprecht, C., Smith, P. J., Hahn, M. G., Urbanowicz, B. R., & Pfrengle, F. (2017). Automated glycan assembly of galactosylated xyloglucan oligosaccharides and their recognition by plant cell wall glycan-directed antibodies. Organic & Biomolecular Chemistry, 15(47), 9996-10000. doi:10.1039/C7OB02605F.

Item is

 

show all hide all

Basic

show hide
Item Permalink: https://hdl.handle.net/11858/00-001M-0000-002E-7C4B-8 Version Permalink: https://hdl.handle.net/21.11116/0000-0000-3573-3
Genre: Journal Article

Files

show Files
hide Files
:
Article.pdf (Publisher version), 2MB
View   Save
File Permalink:
https://hdl.handle.net/11858/00-001M-0000-002E-8B0F-A
Name:
Article.pdf
Description:
-
OA-Status:
Hybrid
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
View
Copyright Date:
-
Copyright Info:
-
License:
http://creativecommons.org/licenses/by/3.0/

Locators

show

Creators

show
hide
 Creators:
Dallabernardina, Pietro1, Author           
Ruprecht, Colin1, Author           
Smith, Peter J., Author
Hahn, Michael G., Author
Urbanowicz, Breeanna R., Author
Pfrengle, Fabian1, Author           
Affiliations:
1Fabian Pfrengle, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863303              

Content

show
hide
Free keywords: Open Access
 Abstract: We report the automated glycan assembly of oligosaccharides related to the plant cell wall hemicellulosic polysaccharide xyloglucan. The synthesis of galactosylated xyloglucan oligosaccharides was enabled by introducing p-methoxybenzyl (PMB) as a temporary protecting group for automated glycan assembly. The generated oligosaccharides were printed as microarrays, and the binding of a collection of xyloglucan-directed monoclonal antibodies (mAbs) to the oligosaccharides was assessed. We also demonstrated that the printed glycans can be further enzymatically modified while appended to the microarray surface by Arabidopsis thaliana xyloglucan xylosyltransferase 2 (AtXXT2).

Details

show
hide
Language(s):
 Dates: 2017-11-212017-12-21
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1039/C7OB02605F
 Degree: -

Event

show

Legal Case

show

Project information

show hide
Project name : -
Grant ID : -
Funding program : Emmy Noether Program (PF850/1-1)
Funding organization : German Research Foundation (DFG)

Source 1

show
hide
Title: Organic & Biomolecular Chemistry
  Other : Organic and Biomolecular Chemistry
  Abbreviation : Org. Biomol. Chem.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Cambridge : Royal Society of Chemistry
Pages: - Volume / Issue: 15 (47) Sequence Number: - Start / End Page: 9996 - 10000 Identifier: ISSN: 1477-0520