Automated glycan assembly of galactosylated xyloglucan oligosaccharides and 
their recognition by plant cell wall glycan-directed antibodies

Dallabernardina, Pietro; Ruprecht, Colin; Smith, Peter J.; Hahn, Michael G.; Urbanowicz, Breeanna R.; Pfrengle, Fabian

doi:10.1039/C7OB02605F

アイテム詳細

登録内容を編集ファイル形式で保存

一時保存へ追加

このアイテムの新しいバージョンが利用可能です:
https://pure.mpg.de/pubman/item/item_2505759_9

詳細要約

公開

学術論文

Automated glycan assembly of galactosylated xyloglucan oligosaccharides and their recognition by plant cell wall glycan-directed antibodies

MPS-Authors

/persons/resource/persons185925

Dallabernardina, Pietro
Fabian Pfrengle, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

/persons/resource/persons97366

Ruprecht, Colin
Fabian Pfrengle, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

/persons/resource/persons185928

Pfrengle, Fabian
Fabian Pfrengle, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

External Resource

There are no locators available

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

フルテキスト (公開)

Article.pdf
(出版社版), 2MB

付随資料 (公開)

There is no public supplementary material available

引用

Dallabernardina, P., Ruprecht, C., Smith, P. J., Hahn, M. G., Urbanowicz, B. R., & Pfrengle, F. (2017). Automated glycan assembly of galactosylated xyloglucan oligosaccharides and their recognition by plant cell wall glycan-directed antibodies. Organic & Biomolecular Chemistry, 15(47), 9996-10000. doi:10.1039/C7OB02605F.

引用: https://hdl.handle.net/11858/00-001M-0000-002E-7C4B-8

要旨

We report the automated glycan assembly of oligosaccharides related to the plant cell wall hemicellulosic polysaccharide xyloglucan. The synthesis of galactosylated xyloglucan oligosaccharides was enabled by introducing p-methoxybenzyl (PMB) as a temporary protecting group for automated glycan assembly. The generated oligosaccharides were printed as microarrays, and the binding of a collection of xyloglucan-directed monoclonal antibodies (mAbs) to the oligosaccharides was assessed. We also demonstrated that the printed glycans can be further enzymatically modified while appended to the microarray surface by Arabidopsis thaliana xyloglucan xylosyltransferase 2 (AtXXT2).