Combined RNAi and localization for functionally dissecting long non-coding RNAs

Chakraborty, Debojyoti; Kappei, Dennis; Theis, Mirko; Nitzsche, Anja; Ding, Li; Paszkowski-Rogacz, Maciej; Surendranath, Vineeth; Berger, Nicolas; Schulz, Herbert; Saar, Kathrin; Hubner, Norbert; Buchholz, Frank

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Combined RNAi and localization for functionally dissecting long non-coding RNAs

Chakraborty, D., Kappei, D., Theis, M., Nitzsche, A., Ding, L., Paszkowski-Rogacz, M., et al. (2012). Combined RNAi and localization for functionally dissecting long non-coding RNAs. Nature Methods, 9(4), 360-362.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0001-07F7-1 Version Permalink: https://hdl.handle.net/21.11116/0000-0001-07F8-0

Genre: Journal Article

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Creators:
Chakraborty, Debojyoti¹, Author
Kappei, Dennis¹, Author
Theis, Mirko¹, Author
Nitzsche, Anja¹, Author
Ding, Li¹, Author
Paszkowski-Rogacz, Maciej¹, Author
Surendranath, Vineeth¹, Author
Berger, Nicolas¹, Author
Schulz, Herbert, Author
Saar, Kathrin², Author
Hubner, Norbert, Author
Buchholz, Frank¹, Author

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1Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society, ou_2340692
2Max Planck Society, ou_persistent13

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Abstract: Whereas methods to comprehensively study cellular roles of protein-coding genes are available, techniques to systematically investigate long noncoding RNAs (lncRNAs), which have been implicated in diverse biological pathways, are limited. Here we report combined knockdown and localization analysis of noncoding RNAs (c-KLAN) that merges functional characterization and localization approaches to study lncRNAs. Using this technique we identified transcripts that regulate mouse embryonic stem cell identity.

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Dates: Date issued: 2012

Publication Status: Issued

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Identifiers: eDoc: 645195
Other: 4649

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Title: Nature Methods

Source Genre: Journal

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Pages: - Volume / Issue: 9 (4) Sequence Number: - Start / End Page: 360 - 362 Identifier: -