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Abstract:
Direct profiling of total lipid extracts on a hybrid LTQ
Orbitrap mass spectrometer by high-resolution survey
spectra clusters species of 11 major lipid classes into 7
groups, which are distinguished by their sum compositions
and could be identified by accurately determined
masses. Rapid acquisition of survey spectra was employed
as a top-down screening tool that, together with the
computational method of principal component analysis,
revealed pronounced perturbations in the abundance of
lipid precursors within the entire series of experiments.
Altered lipid precursors were subsequently identified
either by accurately determined masses or by in-depth
MS/MS characterization that was performed on the same
instrument. Hence, the sensitivity, throughput and robustness
of lipidomics screens were improved without
compromising the accuracy and specificity of molecular
species identification. The top-down lipidomics strategy
lends itself for high-throughput screens complementing
ongoing functional genomics efforts.
