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  Two-colour serial femtosecond crystallography dataset from gadoteridol-derivatized lysozyme for MAD phasing

Gorel, A., Motomura, K., Fukuzawa, H., Doak, R. B., Grünbein, M. L., Hilpert, M., et al. (2017). Two-colour serial femtosecond crystallography dataset from gadoteridol-derivatized lysozyme for MAD phasing. Scientific Data, 4: 170188, pp. 1-11. doi:10.1038/sdata.2017.188.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-3EE5-8 Version Permalink: http://hdl.handle.net/21.11116/0000-0002-C0DA-F
Genre: Journal Article

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Gorel, Alexander1, Author              
Motomura, Koji, Author
Fukuzawa, Hironobu, Author
Doak, R. Bruce1, Author              
Grünbein, Marie Luise1, Author              
Hilpert, Mario1, Author              
Inoue, Ichiro, Author
Kloos, Marco1, Author              
Nass Kovács, Gabriela1, Author              
Nango, Eriko, Author
Nass, Karol1, Author              
Roome, Christopher M.1, Author              
Shoeman, Robert L.1, Author              
Tanaka, Rie, Author
Tono, Kensuke, Author
Foucar, Lutz1, Author              
Joti, Yasumasa, Author
Yabashi, Makina, Author
Iwata, So, Author
Ueda, Kiyoshi, Author
Barends, Thomas R. M.1, Author              Schlichting, Ilme1, Author               more..
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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 Abstract: We provide a detailed description of a gadoteridol-derivatized lysozyme (gadolinium lysozyme) two-colour serial femtosecond crystallography (SFX) dataset for multiple wavelength anomalous dispersion (MAD) structure determination. The data was collected at the Spring-8 Angstrom Compact free-electron LAser (SACLA) facility using a two-colour double-pulse beam to record two diffraction patterns simultaneously in one diffraction image. Gadolinium lysozyme was chosen as a well-established model system that has a very strong anomalous signal. Diffraction patterns from gadolinium lysozyme microcrystals were recorded to a resolution of 1.9 Å in both colours. This dataset is publicly available through the Coherent X-ray Imaging Data Bank (CXIDB) as a resource for algorithm development.

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Language(s): eng - English
 Dates: 2017-09-012017-11-102017-12-12
 Publication Status: Published online
 Pages: 7
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 Rev. Method: Peer
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Title: Scientific Data
  Abbreviation : Sci. Data
Source Genre: Journal
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Publ. Info: London, United Kingdom : Nature Publishing Group
Pages: - Volume / Issue: 4 Sequence Number: 170188 Start / End Page: 1 - 11 Identifier: Other: 2052-4463
CoNE: https://pure.mpg.de/cone/journals/resource/2052-4463