日本語
 
Help Privacy Policy ポリシー/免責事項
  詳細検索ブラウズ

アイテム詳細

登録内容を編集ファイル形式で保存
 
 
ダウンロード電子メール
  Visualizing single-cell secretion dynamics with single protein sensitivity

McDonald, M. P., Gemeinhardt, A., König, K., Piliarik, M., Schaffer, S., Völkl, S., Mackensen, A., & Sandoghdar, V. (2018). Visualizing single-cell secretion dynamics with single protein sensitivity. Nano Letters, 18, 513-519. doi:10.1021/acs.nanolett.7b04494.

Item is

基本情報

表示: 非表示:
アイテムのパーマリンク: https://hdl.handle.net/21.11116/0000-0001-55D9-B 版のパーマリンク: https://hdl.handle.net/21.11116/0000-0006-9FCC-2
資料種別: 学術論文

ファイル

表示: ファイル

関連URL

表示:

作成者

表示:
非表示:
 作成者:
McDonald, Matthew Paul1, 著者           
Gemeinhardt, André1, 著者           
König, Katharina1, 2, 著者           
Piliarik, Marek1, 著者           
Schaffer, Stefanie3, 著者
Völkl, Simon3, 著者
Mackensen, Andreas3, 著者
Sandoghdar, Vahid1, 4, 著者           
所属:
1Sandoghdar Division, Max Planck Institute for the Science of Light, Max Planck Society, ou_2364722              
2International Max Planck Research School, Max Planck Institute for the Science of Light, Max Planck Society, Staudtstraße 2, 91058 Erlangen, DE, ou_2364697              
3 Department of Internal Medicine 5, Hematology and Oncology, Friedrich Alexander University Erlangen-Nuremberg, Ulmenweg 18, 91054 Erlangen, Germany, ou_persistent22              
4Max-Planck-Zentrum für Physik und Medizin, Max Planck Institute for the Science of Light, Max Planck Society, ou_3164414              

内容説明

表示:
非表示:
キーワード: cellular secretion; dynamics; imaging; iSCAT; label-free; single-protein
 要旨: Cellular secretion of proteins into the extracellular environment is an essential mediator of critical biological mechanisms, including cell-to-cell communication, immunological response, targeted delivery, and differentiation. Here, we report a novel methodology that allows for the real-time detection and imaging of single unlabeled proteins that are secreted from individual living cells. This is accomplished via interferometric detection of scattered light (iSCAT) and is demonstrated with Laz388 cells, an Epstein Barr virus (EBV)-transformed B cell line. We find that single Laz388 cells actively secrete IgG antibodies at a rate of the order of 100 molecules per second. Intriguingly, we also find that other proteins and particles spanning ca. 100 kDa-1 MDa are secreted from the Laz388 cells in tandem with IgG antibody release, likely arising from EBV-related viral proteins. The technique is general and, as we show, can also be applied to studying the lysate of a single cell. Our results establish label-free iSCAT imaging as a powerful tool for studying the real-time exchange between cells and their immediate environment with single-protein sensitivity.

資料詳細

表示:
非表示:
言語: eng - English
 日付: 2018-01-10
 出版の状態: 出版
 ページ: 7
 出版情報: -
 目次: -
 査読: -
 識別子(DOI, ISBNなど): DOI: 10.1021/acs.nanolett.7b04494
 学位: -

関連イベント

表示:

訴訟

表示:

Project information

表示:

出版物 1

表示:
非表示:
出版物名: Nano Letters
  省略形 : Nano Lett.
種別: 学術雑誌
 著者・編者:
所属:
出版社, 出版地: Washington, DC : American Chemical Society
ページ: - 巻号: 18 通巻号: - 開始・終了ページ: 513 - 519 識別子(ISBN, ISSN, DOIなど): ISSN: 1530-6984
CoNE: https://pure.mpg.de/cone/journals/resource/110978984570403