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  Efficiency of tissue homogenization via picosecond-infrared laser (PIRL) and classical homogenization as sample preparation step for proteomics

Nimer, R., Kwiatkowski, M., Hansen, N.-O., Miller, R. J. D., & Schlüter, H. (2016). Efficiency of tissue homogenization via picosecond-infrared laser (PIRL) and classical homogenization as sample preparation step for proteomics. Poster presented at 49. Jahrestagung der Deutschen Gesellschaft für Massenspektrometrie (DGMS), Hamburg.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-DE3A-5 Version Permalink: http://hdl.handle.net/21.11116/0000-0001-DE45-8
Genre: Poster

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Posternummer: 18
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Description:
Posternummer: 18

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 Creators:
Nimer, R.1, Author
Kwiatkowski, M.1, Author
Hansen, N.-O.2, Author              
Miller, R. J. D.2, Author              
Schlüter, H.1, Author
Affiliations:
1Universitätsklinikum Hamburg-Eppendorf, ou_persistent22              
2Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_1938288              

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Free keywords: Picosecond Infrared laser (PIRL), desorption impulsive vibrational excitation (DIVE), Top-down proteomic
 Abstract: Introduction: A novel method for ultrafast and soft cold vaporization of tissue via desorption by impulsiv e vibrational excitation (DIVE) using a picosecond - infrared laser (PIRL) has been developed by Dwayne Miller and his group [1]. By irradiating tissue with PIRL proteins are transferred from the tissue into the gas phase within at aerosol plume without chan ging the chemical formula of protein. The chemical composition of the protein is maintained during tissue ablation via PIRL. In this study, the efficiency of tissue homogenization performed with PIRL and with classical homogenization was investigated using two - dimensional electrophoresis (2DE) followed by mass spectrometric analysis (MS) of the tryptic peptides of the separated proteins from the 2DE gel.

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Language(s): eng - English
 Dates: 2016
 Publication Status: Not specified
 Pages: 1
 Publishing info: -
 Table of Contents: -
 Rev. Type: No review
 Identifiers: -
 Degree: -

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Title: 49. Jahrestagung der Deutschen Gesellschaft für Massenspektrometrie (DGMS)
Place of Event: Hamburg
Start-/End Date: 2016-02-28 - 2016-03-02

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