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  A transcriptomics resource reveals a transcriptional transition during ordered sarcomere morphogenesis in flight muscle

Spletter, M. L., Barz, C., Yeroslaviz, A., Zhang, X., Lemke, S. B., Bonnard, A., et al. (2018). A transcriptomics resource reveals a transcriptional transition during ordered sarcomere morphogenesis in flight muscle. eLife, 7: e34058. doi:10.7554/eLife.34058.

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elife-34058-v2.pdf (Publisher version), 14MB
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 Creators:
Spletter, Maria L.1, Author           
Barz, Christiane1, Author           
Yeroslaviz, Assa2, Author           
Zhang, Xu1, Author           
Lemke, Sandra B.1, Author           
Bonnard, Adrien3, Author
Brunner, Erich3, Author
Cardone, Giovanni4, Author           
Basler, Konrad3, Author
Habermann, Bianca H.2, Author           
Schnorrer, Frank1, Author           
Affiliations:
1Schnorrer, Frank / Muscle Dynamics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565168              
2Habermann, Bianca / Computational Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1832284              
3external, ou_persistent22              
4Scientific Service Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565170              

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Free keywords: MYOCYTE ENHANCER FACTOR-2; SKELETAL-MUSCLE; GENE-EXPRESSION; ADULT MYOGENESIS; TRANSGENIC RNAI; DROSOPHILA; MYOFIBRILLOGENESIS; PROTEINS; GROWTH; MEF2Life Sciences & Biomedicine - Other Topics;
 Abstract: Muscles organise pseudo-crystalline arrays of actin, myosin and titin filaments to build force-producing sarcomeres. To study sarcomerogenesis, we have generated a transcriptomics resource of developing Drosophila flight muscles and identified 40 distinct expression profile clusters. Strikingly, most sarcomeric components group in two clusters, which are strongly induced after all myofibrils have been assembled, indicating a transcriptional transition during myofibrillogenesis. Following myofibril assembly, many short sarcomeres are added to each myofibril. Subsequently, all sarcomeres mature, reaching 1.5 mu m diameter and 3.2 mu m length and acquiring stretch-sensitivity. The efficient induction of the transcriptional transition during myofibrillogenesis, including the transcriptional boost of sarcomeric components, requires in part the transcriptional regulator Spalt major. As a consequence of Spalt knock-down, sarcomere maturation is defective and fibers fail to gain stretch-sensitivity. Together, this defines an ordered sarcomere morphogenesis process under precise transcriptional control - a concept that may also apply to vertebrate muscle or heart development.

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Language(s): eng - English
 Dates: 2018
 Publication Status: Published online
 Pages: 34
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000435436400001
DOI: 10.7554/eLife.34058
 Degree: -

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Project name : H2020 European Research Council (ERC Grant 310939)
Grant ID : 310939
Funding program : Horizon 2020 (H2020)
Funding organization : European Commission (EC)

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Title: eLife
Source Genre: Journal
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Publ. Info: Cambridge : eLife Sciences Publications
Pages: - Volume / Issue: 7 Sequence Number: e34058 Start / End Page: - Identifier: ISSN: 2050-084X
CoNE: https://pure.mpg.de/cone/journals/resource/2050-084X